Rabbit anti-Human GRM1 Polyclonal Antibody | anti-GRM1 antibody

GRM1, CT (GRM1, GPRC1A, MGLUR1, Metabotropic glutamate receptor 1) (FITC)

Cat. Num. AAA6304495

• Gene Names: GRM1; MGLU1; SCA44; GPRC1A; MGLUR1; SCAR13; PPP1R85
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GRM1; Polyclonal Antibody; CT (GRM1; GPRC1A; MGLUR1; Metabotropic glutamate receptor 1) (FITC); Metabotropic glutamate receptor 1; anti-GRM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GRM1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GRM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1094-1123 amino acids from the C-terminal region of human GRM1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane). usingMBS641468. This demonstrates the GRM1 antibody detected the GRM1 protein (arrow).)

Related Product Information for anti-GRM1 antibody

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. The canonical alpha isoform of the metabotropic glutamate receptor 1 gene is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. Alternative splicing results in multiple transcript variants encoding distinct isoforms; some of which may have distinct functions. [provided by RefSeq].

Product Categories/Family for anti-GRM1 antibody

Antibodies; G Protein Receptors

References

Jiang, Y., et al. J. Biol. Chem. 285(43):33463-33474(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Gong, P., et al. J. Mol. Neurosci. 42(1):120-126(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Boer, K., et al. Brain Res. 1324, 24-33 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 166999223
• NCBI GeneID: 2911
• UniProt Accession #: Q13255
• NCBI Official Synonym Full Names: glutamate metabotropic receptor 1
• NCBI Official Symbol: GRM1
• NCBI Official Synonym Symbols: MGLU1; SCA44; GPRC1A; MGLUR1; SCAR13; PPP1R85
• NCBI Protein Information: metabotropic glutamate receptor 1
• UniProt Protein Name: Metabotropic glutamate receptor 1
• Protein Family: Metabotropic glutamate receptor
• UniProt Gene Name: GRM1
• UniProt Synonym Gene Names: GPRC1A; MGLUR1; mGluR1
• UniProt Entry Name: GRM1_HUMAN

NCBI Description

This gene encodes a metabotropic glutamate receptor that functions by activating phospholipase C. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The canonical alpha isoform of the encoded protein is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. This gene may be associated with many disease states, including schizophrenia, bipolar disorder, depression, and breast cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]

Uniprot Description

mGluR1: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol- calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP. Belongs to the G-protein coupled receptor 3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 3; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q24

Cellular Component: integral to plasma membrane; postsynaptic density; dendrite; plasma membrane; nucleus

Molecular Function: G-protein coupled receptor activity; protein binding; glutamate receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; synaptic transmission; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of synaptic transmission, glutamatergic; activation of MAPKK activity; activation of MAPK activity; protein kinase C activation; metabotropic glutamate receptor signaling pathway; locomotory behavior; regulation of sensory perception of pain; sensory perception of pain

Disease: Spinocerebellar Ataxia, Autosomal Recessive 13