Goat GRIK1 / GLUR5 Polyclonal Antibody | anti-GRIK1 antibody

Goat Polyclonal to Human GRIK1 / GLUR5

Cat. Num. AAA244418

• Gene Names: GRIK1; EAA3; EEA3; GLR5; GLUR5; GluK1
• Reactivity: Human, Monkey, Mouse, Rat, Horse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GRIK1 / GLUR5; Polyclonal Antibody; Goat Polyclonal to Human GRIK1 / GLUR5; Anti-GRIK1 / GLUR5 Antibody (Internal) IHC-plus; GRIK1; GluR-5; GLUR5; EEA3; GluK1; Glutamate receptor 5; EAA3; GLR5; Human GRIK1; anti-GRIK1 antibody

• Host: Goat
• Reactivity: Human, Monkey, Mouse, Rat, Horse
• Clonality: Polyclonal
• Specificity: Human GRIK1. This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 918

• Applicable Applications for anti-GRIK1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:8000), IHC-P (3.75 ug/ml), WB (1:8000, 1 - 3 ug/ml)
• Target Species: Human
• Presentation: TBS, pH 7.3, 0.02% sodium Azide, 0.5% BSA
• Epitope: aa878-889
• Immunogen: Peptide with sequence QCKQTHPTNSTS, from the internal region (near the C-Terminus) of the protein sequence according to NP_000821.1NP_783300.1.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C. Minimize freezing and thawing.

Product Categories/Family for anti-GRIK1 antibody

Family: Ion Channel; Subfamily: Glutamate receptor - ionotropic (NMDA receptor)

NCBI and Uniprot Product Information

• NCBI GI #: 4504117
• NCBI GeneID: 2897
• NCBI Accession #: NP_000821.1
• NCBI GenBank Nucleotide #: NM_000830.3
• UniProt Accession #: P39086; Q13001; Q86SU9
• NCBI Official Full Name: glutamate receptor ionotropic, kainate 1 isoform 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, kainate 1
• NCBI Official Symbol: GRIK1
• NCBI Official Synonym Symbols: EAA3; EEA3; GLR5; GLUR5; GluK1
• NCBI Protein Information: glutamate receptor ionotropic, kainate 1; gluR-5; glutamate receptor 5; excitatory amino acid receptor 3
• UniProt Protein Name: Glutamate receptor ionotropic, kainate 1
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIK1
• UniProt Synonym Gene Names: GLUR5; GluK1; EAA3; GluR-5; GluR5
• UniProt Entry Name: GRIK1_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR5: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: presynaptic membrane; postsynaptic membrane; kainate selective glutamate receptor complex; integral to plasma membrane; dendrite; plasma membrane; cell junction

Molecular Function: kainate selective glutamate receptor activity; extracellular-glutamate-gated ion channel activity

Biological Process: nervous system development; synaptic transmission, glutamatergic; central nervous system development; regulation of synaptic transmission, glutamatergic; glutamate signaling pathway; positive regulation of synaptic transmission, GABAergic; negative regulation of synaptic transmission, glutamatergic; synaptic transmission; regulation of inhibitory postsynaptic membrane potential; behavioral response to pain; ionotropic glutamate receptor signaling pathway; transport; adult behavior; regulation of excitatory postsynaptic membrane potential; positive regulation of gamma-aminobutyric acid secretion