Rabbit Grik1 Polyclonal Antibody | anti-GRIK1 antibody

Grik1 Antibody

Cat. Num. AAA151021

• Gene Names: GRIK1; EAA3; EEA3; GLR5; GLUR5; GluK1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Grik1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Grik1; Polyclonal Antibody; Grik1 Antibody; EAA3; EEA3; GLR5; GLUR5; GluK1; Glutamate receptor ionotropic; kainate 1; Excitatory amino acid receptor 3; glutamate receptor; ionotropic; anti-GRIK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Grik1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 918

• Applicable Applications for anti-GRIK1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Grik1 antibody can be used for detection of Grik1 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Grik1 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Grik1.
• Buffer: Grik1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Grik1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Grik1 in rat brain tissue lysate with Grik1 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human brain tissue using Grik1 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Grik1 in Human Brain cells with Grik1 antibody at 20 μg/mL.)

Related Product Information for anti-GRIK1 antibody

Grik1 Antibody: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik1, also known as glutamate receptor 5, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Grik1 is expressed in GABAergic interneurons of the hippocampus and are thought to participate in the formation of various subtypes of kainate receptors with Grik2 and KA2. Stimulation of Grik1 leads to intracellular calcium release and activation of protein kinase C. Excessive activation has been associated with psychiatric, neurological and neurodegenerative diseases. Numerous isoforms of Grik1 are known to exist and may be subject to RNA editing within the second transmembrane domain, which is thought to alter the properties of ion flow.

NCBI and Uniprot Product Information

• NCBI GI #: 729597
• NCBI GeneID: 2897
• UniProt Accession #: P39086; Q13001; Q86SU9
• Molecular Weight: 102,631 Da
• NCBI Official Full Name: Glutamate receptor ionotropic, kainate 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, kainate 1
• NCBI Official Symbol: GRIK1
• NCBI Official Synonym Symbols: EAA3; EEA3; GLR5; GLUR5; GluK1
• NCBI Protein Information: glutamate receptor ionotropic, kainate 1; gluR-5; glutamate receptor 5; excitatory amino acid receptor 3
• UniProt Protein Name: Glutamate receptor ionotropic, kainate 1
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIK1
• UniProt Synonym Gene Names: GLUR5; GluK1; EAA3; GluR-5; GluR5
• UniProt Entry Name: GRIK1_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR5: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: presynaptic membrane; postsynaptic membrane; kainate selective glutamate receptor complex; integral to plasma membrane; dendrite; plasma membrane; cell junction

Molecular Function: kainate selective glutamate receptor activity; extracellular-glutamate-gated ion channel activity

Biological Process: nervous system development; synaptic transmission, glutamatergic; regulation of synaptic transmission, glutamatergic; central nervous system development; glutamate signaling pathway; positive regulation of synaptic transmission, GABAergic; negative regulation of synaptic transmission, glutamatergic; synaptic transmission; regulation of inhibitory postsynaptic membrane potential; behavioral response to pain; transport; ionotropic glutamate receptor signaling pathway; adult behavior; regulation of excitatory postsynaptic membrane potential; positive regulation of gamma-aminobutyric acid secretion