Typical Testing Data/Standard Curve (for reference only)
Mouse Cytoglobin ELISA Kit | CYGB elisa kit
Mouse CYGB (Cytoglobin) ELISA kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CYGB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CYGBand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CYGB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CYGB.You can calculate the concentration of CYGBin the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a globin protein found in vertebrate cells. The encoded protein is described as a hexacoordinate hemoglobin which binds ligand differently from the pentacoordinate hemoglobins involved in oxygen transport, and may be involved in protection during oxidative stress. This gene is located on chromosome 17 in the same region as a retinal gene which is mutated in progressive rod-cone degeneration, but in the opposite orientation. [provided by RefSeq, Jan 2012]
Uniprot Description
CYGB: May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer. Belongs to the globin family.
Chromosomal Location of Human Ortholog: 17q25
Cellular Component: cell soma; cytosol; neuron projection
Molecular Function: catalase activity; fatty-acid peroxidase activity; heme binding; iron ion binding; nitric oxide dioxygenase activity; oxygen binding; oxygen transporter activity; peroxidase activity; protein binding
Biological Process: fatty acid oxidation; negative regulation of collagen biosynthetic process; nitric oxide metabolic process; oxygen transport; regulation of nitric-oxide synthase activity; response to hypoxia; response to oxidative stress
Research Articles on CYGB
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Product Notes
The Mouse CYGB cygb (Catalog #AAA2533462) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533462 ELISA Kit recognizes Mouse CYGB. It is sometimes possible for the material contained within the vial of "Cytoglobin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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