Rabbit anti-Human, Mouse GRIA4 Polyclonal Antibody | anti-GRIA4 antibody

GRIA4, ID (GRIA4, GLUR4, Glutamate receptor 4, AMPA-selective glutamate receptor 4, GluR-D, Glutamate receptor ionotropic, AMPA 4) (FITC)

Cat. Num. AAA6304363

• Gene Names: GRIA4; GLUR4; GLURD; GluA4; GLUR4C
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GRIA4; Polyclonal Antibody; ID (GRIA4; GLUR4; Glutamate receptor 4; AMPA-selective glutamate receptor 4; GluR-D; Glutamate receptor ionotropic; AMPA 4) (FITC); AMPA 4; anti-GRIA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GRIA4 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GRIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-325 amino acids from the Central region of human GRIA4.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GRIA4 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GRIA4 antibody detected the GRIA4 protein (arrow).)

Western Blot (WB)

(GRIA4 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the GRIA4 antibody detected the GRIA4 protein (arrow).)

Immunohistochemistry (IHC)

(GRIA4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GRIA4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-GRIA4 antibody

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq].

Product Categories/Family for anti-GRIA4 antibody

Antibodies; Receptors

References

Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009). Volpi, S., et al. J Clin Psychiatry 70(6):801-809(2009). Kessels, H.W., et al. Neuron 61(3):340-350(2009). Arai, S., et al. Psychiatr. Genet. 19(1):6-13(2009). Aruscavage, P.J., et al. RNA 6(2):257-269(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 164419734
• NCBI GeneID: 2893
• NCBI Accession #: NP_000820.3
• NCBI GenBank Nucleotide #: NM_000829.3
• UniProt Accession #: P48058; Q86XE8
• Molecular Weight: 100,871 Da
• NCBI Official Full Name: glutamate receptor 4 isoform 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, AMPA 4
• NCBI Official Symbol: GRIA4
• NCBI Official Synonym Symbols: GLUR4; GLURD; GluA4; GLUR4C
• NCBI Protein Information: glutamate receptor 4; gluR-4; gluR-D; AMPA-selective glutamate receptor 4; glutamate receptor, ionotrophic, AMPA 4
• UniProt Protein Name: Glutamate receptor 4
• Protein Family: Glutamate receptor
• UniProt Gene Name: GRIA4
• UniProt Synonym Gene Names: GLUR4; GluR-4; GluR4; GluA4
• UniProt Entry Name: GRIA4_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR4: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA(quisqualate) > glutamate > kainate. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 11q22

Cellular Component: postsynaptic membrane; dendrite; plasma membrane; cell junction; vesicle

Molecular Function: extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity

Biological Process: synaptic transmission; synaptic transmission, glutamatergic; glutamate signaling pathway; transport; ionotropic glutamate receptor signaling pathway