Rabbit anti-Human, Mouse GRB10 Polyclonal Antibody | anti-GRB10 antibody

GRB10 (Growth Factor Receptor-bound Protein 10, GRB-IR, Grb-10, IRBP, KIAA0207, MEG1, RSS) (FITC)

Cat. Num. AAA6419151

• Gene Names: GRB10; RSS; IRBP; MEG1; GRB-IR; Grb-10
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: GRB10; Polyclonal Antibody; GRB10 (Growth Factor Receptor-bound Protein 10; GRB-IR; Grb-10; IRBP; KIAA0207; MEG1; RSS) (FITC); anti-GRB10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes GRB10. Species Crossreactivity: human, mouse
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-GRB10 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human GRB10.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of mouse brain tissue, using GRB10 antibody.)

Product Categories/Family for anti-GRB10 antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 48762693
• NCBI GeneID: 2887
• NCBI Accession #: NP_001001549.1
• NCBI GenBank Nucleotide #: NM_001001549.2
• UniProt Accession #: Q13322; O00427; O00701; O75222; Q92606; Q92907; Q92948; A4D258; A7VJ95; A8K0E6; D3DVM9
• Molecular Weight: 67,231 Da
• NCBI Official Full Name: growth factor receptor-bound protein 10 isoform b
• NCBI Official Synonym Full Names: growth factor receptor-bound protein 10
• NCBI Official Symbol: GRB10
• NCBI Official Synonym Symbols: RSS; IRBP; MEG1; GRB-IR; Grb-10
• NCBI Protein Information: growth factor receptor-bound protein 10; GRB10 adapter protein; GRB10 adaptor protein; maternally expressed gene 1; insulin receptor-binding protein Grb-IR
• UniProt Protein Name: Growth factor receptor-bound protein 10
• Protein Family: Growth factor receptor-bound protein
• UniProt Gene Name: GRB10
• UniProt Synonym Gene Names: GRBIR; KIAA0207
• UniProt Entry Name: GRB10_HUMAN

NCBI Description

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]

Uniprot Description

GRB10: an adaptor protein that inhibits insulin-stimulated IRS-PI (3)K-Akt signaling pathway by disrupting the association of IRS with the insulin receptor. Also binds activated PDGFR and EGFR. Preferentially localizes to the mitochondria, modulating the anti-apoptotic activity of mitochondrial Raf-1. Can translocate to the plasma membrane and lamellipodia. Three alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; insulin receptor binding

Biological Process: negative regulation of glucose import; negative regulation of Wnt receptor signaling pathway; insulin-like growth factor receptor signaling pathway; insulin receptor signaling pathway; negative regulation of phosphorylation; negative regulation of insulin receptor signaling pathway; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of phosphorylation; response to insulin stimulus; negative regulation of glycogen biosynthetic process