Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat HSPb6 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit anti-Rat Heat Shock Protein Beta 6 (HSPb6) Polyclonal Antibody | anti-HSPb6 antibody
Polyclonal Antibody to Heat Shock Protein Beta 6 (HSPb6)
Gene Names
Hspb6; p20; Hsp20
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Heat Shock Protein Beta 6 (HSPb6); Polyclonal Antibody; Polyclonal Antibody to Heat Shock Protein Beta 6 (HSPb6); anti-HSPb6 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HSPb6. It has been selected for its ability to recognize HSPb6 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-MEIRVPVQPS WLRRASAPLP GFSTPGRLFD QRFGEGLLEA ELASLCPAAI APYYLRAPSV ALPTAQVPTD PGYFSVLLDV KHFSPEEISV KVVGDHVEVH ARHEERPDEH GFIAREFHRR YRLPPGVDPA AVTSALSPEG VLSIQATPAS AQASLPSPPA AK
MGHHHHHHSGSEF-MEIRVPVQPS WLRRASAPLP GFSTPGRLFD QRFGEGLLEA ELASLCPAAI APYYLRAPSV ALPTAQVPTD PGYFSVLLDV KHFSPEEISV KVVGDHVEVH ARHEERPDEH GFIAREFHRR YRLPPGVDPA AVTSALSPEG VLSIQATPAS AQASLPSPPA AK
Sequence Length
162
Applicable Applications for anti-HSPb6 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant HSPb6 (Met1~Lys162) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2076364
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat HSPb6 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat HSPb6 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,505 Da
NCBI Official Full Name
heat shock protein beta-6
NCBI Official Synonym Full Names
heat shock protein family B (small) member 6
NCBI Official Symbol
Hspb6
NCBI Official Synonym Symbols
p20; Hsp20
NCBI Protein Information
heat shock protein beta-6
UniProt Protein Name
Heat shock protein beta-6
Protein Family
UniProt Gene Name
Hspb6
UniProt Synonym Gene Names
HspB6
NCBI Description
may play a role in muscle contraction [RGD, Feb 2006]
Uniprot Description
HSP20: a small heat shock protein of the HSP20 family.
Protein type: Chaperone; Heat shock protein
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: cytoplasm; extracellular region; nucleus
Molecular Function: chaperone binding; protein homodimerization activity; structural constituent of eye lens; unfolded protein binding
Biological Process: negative regulation of cardiac muscle cell apoptotic process; positive regulation of angiogenesis; regulation of muscle contraction
Research Articles on HSPb6
Similar Products
Product Notes
The HSPb6 hspb6 (Catalog #AAA2003854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Heat Shock Protein Beta 6 (HSPb6) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock Protein Beta 6 (HSPb6) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HSPb6 hspb6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-MEIRVP VQPS WLRRASAPLP GFSTPGRLFD QRFGEGLLEA ELASLCPAAI APYYLRAPSV ALPTAQVPTD PGYFSVLLDV KHFSPEEISV KVVGDHVEVH ARHEERPDEH GFIAREFHRR YRLPPGVDPA AVTSALSPEG VLSIQATPAS AQASLPSPPA AK. It is sometimes possible for the material contained within the vial of "Heat Shock Protein Beta 6 (HSPb6), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
