General Transcription Factor IIH Recombinant Protein | GTF2H5 recombinant protein

Recombinant General Transcription Factor IIH, Polypeptide 5 (GTF2H5)

Cat. Num. AAA2034432

• Gene Names: GTF2H2; p44; BTF2; TFIIH; BTF2P44; T-BTF2P44
• Reactivity: Human
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: General Transcription Factor IIH; Recombinant General Transcription Factor IIH; Polypeptide 5 (GTF2H5); TTD; TFB5; TGF2H5; TTD-A; TTDA; C6orf175; DNA Repair Syndrome Trichothiodystrophy Group A; TFIIH basal transcription factor complex TTD-A subunit; GTF2H5 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: >95%
• Sequence Length: 395

• Applicable Applications for GTF2H5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Fragment: Met1~Lys71
• Tag: Prokaryotic expression
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6681762
• NCBI GeneID: 2966
• NCBI Accession #: NP_001506.1
• NCBI GenBank Nucleotide #: NM_001515.3
• Molecular Weight: 11.8kDa
• NCBI Official Full Name: general transcription factor IIH subunit 2
• NCBI Official Synonym Full Names: general transcription factor IIH subunit 2
• NCBI Official Symbol: GTF2H2
• NCBI Official Synonym Symbols: p44; BTF2; TFIIH; BTF2P44; T-BTF2P44
• NCBI Protein Information: general transcription factor IIH subunit 2
• UniProt Protein Name: General transcription factor IIH subunit 2
• Protein Family: General transcription factor
• UniProt Gene Name: GTF2H2
• UniProt Synonym Gene Names: BTF2P44; BTF2 p44
• UniProt Entry Name: TF2H2_HUMAN

NCBI Description

This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs; however, no sequence data is currently available for the centromeric copy of the gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GTF2H2: Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C- terminus is required for a successful escape of RNAP II form the promoter. Belongs to the GTF2H2 family. 1 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Transcription factor; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: holo TFIIH complex; nucleoplasm; nucleus; transcription factor TFIID complex

Molecular Function: DNA-dependent ATPase activity; nucleic acid binding; protein binding; protein kinase activity; protein N-terminus binding; RNA polymerase subunit kinase activity; translation factor activity, nucleic acid binding

Biological Process: G-protein coupled receptor internalization; mRNA capping; nucleotide-excision repair; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; regulation of transcription from RNA polymerase II promoter; response to UV; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; termination of RNA polymerase I transcription; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair