Rabbit anti-Human Gq, 11 alpha CT Polyclonal Antibody | anti-GNAO1 antibody

Rabbit anti-Human Gq, 11 alpha, CT (GNA11, GA11)

Cat. Num. AAA611422

• Gene Names: GNAO1; GNAO; G-ALPHA-o
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography
• Synonyms: Gq; 11 alpha CT; Polyclonal Antibody; Rabbit anti-Human Gq; 11 alpha; CT (GNA11; GA11); Anti -Gq; anti-GNAO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Gqa and G11a. Does not recognize other non-pertussis toxin-sensitive G proteins.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

• Applicable Applications for anti-GNAO1 antibody: Western Blot (WB), Immunoprecipitation (IP); Other applications have not tested
• Application Notes: Western Blot: 1:500–1:2000 detects Gqa and G11a in 20ug of rat brain microsomal preparation. A previous lot detected Gqa and G11a in 20ug mouse brain microsomes and membranes and Gq/11a in 10ug of mouse 3T3/NIH fibroblast soluble plasma membranes. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS611422. Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate Gqa and G11a.; ; Recommended Secondary Antibodies:; MBS616614: IgG, X-Adsorbed (HRP) Pab Gt x Rb; MBS615892: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb; MBS615214: IgG, H&L (HRP) Pab Gt x Rb
• Immunogen: Synthetic peptide corresponding to aa350- 359, QLNLKEYNLV of G11a or Gqa (KLH). This sequence is highly conserved among species.
• Species Sequence Homology: Mammals
• Western Blot Protocol: 1. Perform SDS-PAGE on microsome or membrane sample and transfer the proteins to nitrocellulose. Wash the blotted nitrocellulose twice with water.; 2. Block the blotted nitrocellulose in freshly prepared PBS containing 3% nonfat dry milk (PBS-MLK) for 30 minutes at RT with constant agitation.; 3. Incubate the nitrocellulose with MBS611422, diluted in freshly prepared PBS-MLK overnight with agitation at 4°C.; 4. Wash the nitrocellulose twice with water.; 5. Incubate the nitrocellulose in the secondary reagent of choice (lgG (HRP) goat anti-rabbit)* in PBS-MLK for 1.5 hours at RT with agitation.; 6. Wash the nitrocellulose with water twice.; 7. Wash the nitrocellulose in PBS-O.OS% Tween-20 for 3-5 minutes.; 8. Rinse the nitrocellulose in 4-5 changes of water.; 9. Use detection method of choice (enhanced chemiluminescence was used).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS611422 (1:2000). Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.)

Product Categories/Family for anti-GNAO1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 162461738
• NCBI GeneID: 2775
• NCBI Accession #: NM_002072
• NCBI GenBank Nucleotide #: NM_138736.2
• Molecular Weight: 40,051 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(o) subunit alpha isoform b
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
• NCBI Official Symbol: GNAO1
• NCBI Official Synonym Symbols: GNAO; G-ALPHA-o
• NCBI Protein Information: guanine nucleotide-binding protein G(o) subunit alpha; guanine nucleotide-binding protein G(o) subunit alpha
• UniProt Protein Name: Guanine nucleotide-binding protein G(o) subunit alpha
• UniProt Gene Name: GNAO1
• UniProt Entry Name: GNAO_HUMAN

NCBI Description

The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]

Uniprot Description

G-alpha(o): Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14. Interacts with RGS14. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Belongs to the G-alpha family. G(i/o/t/z) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, heterotrimeric alpha G((i/o/t/z)); G protein; G protein, heterotrimeric

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: neuron projection; plasma membrane; heterotrimeric G-protein complex

Molecular Function: corticotropin-releasing hormone receptor 1 binding; GTPase activity; signal transducer activity; GTP binding; mu-type opioid receptor binding; metal ion binding; G-protein beta/gamma-subunit binding; GTPase activating protein binding; metabotropic serotonin receptor binding

Biological Process: response to drug; negative regulation of calcium ion transport; metabolic process; response to morphine; locomotory behavior; regulation of heart contraction; G-protein signaling, coupled to cAMP nucleotide second messenger; muscle contraction; response to hydrogen peroxide; response to cytokine stimulus; forebrain development; dopamine receptor signaling pathway; neurite development; positive regulation of GTPase activity; aging

Disease: Epileptic Encephalopathy, Early Infantile, 17