Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GPX8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Rabbit GPX8 Polyclonal Antibody | anti-GPX8 antibody
GPX8 Rabbit pAb
Gene Names
GPX8; GPx-8; UNQ847; EPLA847; GSHPx-8
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
GPX8; Polyclonal Antibody; GPX8 Rabbit pAb; GPx-8; UNQ847; EPLA847; GSHPx-8; anti-GPX8 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-GPX8 antibody
Western Blot (WB)
Application Notes
WB: 1:1000-1:2000
Immunogen
Recombinant protein of human GPX8.
Positive Samples
U2OS, MCF7, RD, Mouse heart, Rat lung
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GPX8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GPX8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,881 Da
NCBI Official Full Name
probable glutathione peroxidase 8 isoform a
NCBI Official Synonym Full Names
glutathione peroxidase 8 (putative)
NCBI Official Symbol
GPX8
NCBI Official Synonym Symbols
GPx-8; UNQ847; EPLA847; GSHPx-8
NCBI Protein Information
probable glutathione peroxidase 8
UniProt Protein Name
Probable glutathione peroxidase 8
Protein Family
UniProt Gene Name
GPX8
UniProt Synonym Gene Names
GPx-8; GSHPx-8
UniProt Entry Name
GPX8_HUMAN
Uniprot Description
GPX8: Belongs to the glutathione peroxidase family.
Protein type: EC 1.11.1.9; Oxidoreductase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q11.2
Cellular Component: endoplasmic reticulum lumen; integral to membrane
Molecular Function: glutathione peroxidase activity; peroxidase activity
Biological Process: response to reactive oxygen species
Research Articles on GPX8
1. GPX8 is transcriptionally regulated by HIFalpha and modulates growth factor signaling in HeLa cells.
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Product Notes
The GPX8 gpx8 (Catalog #AAA9143656) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPX8 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPX8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-1:2000. Researchers should empirically determine the suitability of the GPX8 gpx8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPX8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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