Rabbit GPX2 Polyclonal Antibody | anti-GPX2 antibody

GPX2 Polyclonal Antibody

Cat. Num. AAA9432820

• Gene Names: GPX2; GPRP; GPx-2; GI-GPx; GPRP-2; GPx-GI; GSHPx-2; GSHPX-GI
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: GPX2; Polyclonal Antibody; GPX2 Polyclonal Antibody; GI-GPx; GPRP; GPRP-2; GPx-2; GPx-GI; GSHPX-GI; GSHPx-2; glutathione peroxidase 2; anti-GPX2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 50% glycerol, pH7.4

• Applicable Applications for anti-GPX2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant fusion protein of human GPX2 (NP_002074.2).
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using GPX2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using GPX2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat stomach using GPX2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse stomach using GPX2 antibody.)

Related Product Information for anti-GPX2 antibody

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract (also in liver in human), is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Overexpression of this gene is associated with increased differentiation and proliferation in colorectal cancer. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

Product Categories/Family for anti-GPX2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 32967607
• NCBI GeneID: 2877
• NCBI Accession #: NP_002074.2
• NCBI GenBank Nucleotide #: NM_002083.3
• UniProt Accession #: P18283; Q6PJ52; Q8WWI7; Q9NRP9
• Molecular Weight: 21,954 Da
• NCBI Official Full Name: glutathione peroxidase 2
• NCBI Official Synonym Full Names: glutathione peroxidase 2
• NCBI Official Symbol: GPX2
• NCBI Official Synonym Symbols: GPRP; GPx-2; GI-GPx; GPRP-2; GPx-GI; GSHPx-2; GSHPX-GI
• NCBI Protein Information: glutathione peroxidase 2; gastrointestinal glutathione peroxidase; glutathione peroxidase 2 (gastrointestinal); glutathione peroxidase-related protein 2
• UniProt Protein Name: Glutathione peroxidase 2
• Protein Family: Glutathione peroxidase
• UniProt Gene Name: GPX2
• UniProt Synonym Gene Names: GPx-2; GSHPx-2; GPx-GI; GSHPx-GI; GPRP-2
• UniProt Entry Name: GPX2_HUMAN

NCBI Description

This gene is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. The protein encoded by this locus contains a selenocysteine (Sec) residue encoded by the UGA codon, which normally signals translation termination. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

Uniprot Description

GPX2: Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors. Belongs to the glutathione peroxidase family.

Protein type: EC 1.11.1.9; Oxidoreductase; Other Amino Acids Metabolism - glutathione; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: cytoplasm; cytosol

Molecular Function: electron carrier activity; glutathione peroxidase activity

Biological Process: response to reactive oxygen species; response to symbiotic bacterium; negative regulation of inflammatory response to antigenic stimulus; thermoregulation; interaction with symbiont