Western Blot (WB)
((0.5g/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat GPR81/FKSG80 Polyclonal Antibody | anti-GPR81 antibody
Goat anti-GPR81/FKSG80 Antibody
Gene Names
HCAR1; HCA1; GPR81; LACR1; FKSG80; GPR104; TAGPCR; TA-GPCR
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
GPR81/FKSG80; Polyclonal Antibody; Goat anti-GPR81/FKSG80 Antibody; GPR81; G protein-coupled receptor 81; FKSG80; GPR104; TA-GPCR; G protein-coupled receptor 104; T-cell activation G protein-coupled receptor; putative chemokine receptor; GPR81 antibody; G protein-coupled receptor 81 antibody; FKSG80 antibody; GPR104 antibody; TA-GPCR antibody; G protein-coupled receptor 104 antibody; T-cell activation G protein-coupled receptor antibody; putative chemokine receptor antibody; GPR81 / FKSG80; anti-GPR81 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QQLARQARMKKATR
Sequence Length
346
Applicable Applications for anti-GPR81 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Immunogen
Peptide with sequence C-QQLARQARMKKATR, from the Internal region of the protein sequence according to NP_115943.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.5g/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5g/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,295 Da
NCBI Official Full Name
hydroxycarboxylic acid receptor 1
NCBI Official Synonym Full Names
hydroxycarboxylic acid receptor 1
NCBI Official Symbol
HCAR1
NCBI Official Synonym Symbols
HCA1; GPR81; LACR1; FKSG80; GPR104; TAGPCR; TA-GPCR
NCBI Protein Information
hydroxycarboxylic acid receptor 1
UniProt Protein Name
Hydroxycarboxylic acid receptor 1
UniProt Gene Name
HCAR1
UniProt Synonym Gene Names
GPR104; GPR81; HCA1
UniProt Entry Name
HCAR1_HUMAN
NCBI Description
G protein-coupled receptors (GPCRs, or GPRs), such as GPR81, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.[supplied by OMIM, Feb 2005]
Uniprot Description
Gpr81: Acts as a receptor for L-lactate and mediates its anti- lipolytic effect through a G(i)-protein-mediated pathway. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: integral to membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of defense response to virus by host
Research Articles on GPR81
Similar Products
Product Notes
The GPR81 hcar1 (Catalog #AAA421751) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GPR81/FKSG80 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's GPR81/FKSG80 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Researchers should empirically determine the suitability of the GPR81 hcar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QQLARQARMK KATR. It is sometimes possible for the material contained within the vial of "GPR81/FKSG80, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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