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Western Blot (WB) (Western blot analysis of extracts from mouse brain tissue sample, using GPR8 Antibody.)

Rabbit anti-Human, Mouse GPR8 Polyclonal Antibody | anti-GPR8 antibody

GPR8 Antibody

Gene Names
NPBWR2; GPR8
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GPR8; Polyclonal Antibody; GPR8 Antibody; G protein coupled receptor 8; Neuropeptides B/W receptor type 2; NPBWR2; anti-GPR8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
GPR8 antibody detects endogenous levels of total GPR8
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
333
Applicable Applications for anti-GPR8 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Tissue Specificity
Detected at high levels in caudate nucleus, hyppocampus and amygdala; at moderate levels in the adult brain, thalamus, parietal cortex, pituitary gland, adrenal gland and lymph nodes.
Conjugation
Unconjugated

Western Blot (WB)

(Western blot analysis of extracts from mouse brain tissue sample, using GPR8 Antibody.)

Western Blot (WB) (Western blot analysis of extracts from mouse brain tissue sample, using GPR8 Antibody.)

Immunohistochemistry (IHC)

(MBS9604278 at 1/100 staining human prostate cancer tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hou)

Immunohistochemistry (IHC) (MBS9604278 at 1/100 staining human prostate cancer tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hou)

Western Blot (WB)

(Western blot analysis of GPR8 Antibody expression in Human brain tissue lysates.The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of GPR8 Antibody expression in Human brain tissue lysates.The lane on the left is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9604278 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9604278 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-GPR8 antibody
Function: Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals.
Similarity: Belongs to the G-protein coupled receptor 1 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 37 kDa
Predicted: 37 kDa
NCBI Official Full Name
neuropeptides B/W receptor type 2
NCBI Official Synonym Full Names
neuropeptides B and W receptor 2
NCBI Official Symbol
NPBWR2
NCBI Official Synonym Symbols
GPR8
NCBI Protein Information
neuropeptides B/W receptor type 2
UniProt Protein Name
Neuropeptides B/W receptor type 2
UniProt Gene Name
NPBWR2
UniProt Synonym Gene Names
GPR8

NCBI Description

The protein encoded by this gene is an integral membrane protein and G protein-coupled receptor. The encoded protein is similar in sequence to another G protein-coupled receptor (GPR7), and it is structurally similar to opioid and somatostatin receptors. This protein binds neuropeptides B and W. This gene is intronless and is expressed primarily in the frontal cortex of the brain. [provided by RefSeq, Jul 2008]

Uniprot Description

Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals.

Research Articles on GPR8

Similar Products

Product Notes

The GPR8 npbwr2 (Catalog #AAA9604278) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR8 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GPR8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the GPR8 npbwr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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