Rabbit anti-Human GPR34 Polyclonal Antibody | anti-GPR34 antibody

GPR34, ID (GPR34, Probable G-protein coupled receptor 34) (APC)

Cat. Num. AAA6303910

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPR34; Polyclonal Antibody; ID (GPR34; Probable G-protein coupled receptor 34) (APC); Probable G-protein coupled receptor 34; anti-GPR34 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-GPR34 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GPR34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-269 amino acids from the Central region of human GPR34.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GPR34 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GPR34 antibody detected the GPR34 protein (arrow).)

Immunohistochemistry (IHC)

(GPR34 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPR34 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-GPR34 antibody

G protein-coupled receptors (GPCRs), such as GPR34, are integral membrane proteins containing 7 putative transmembrane domains (TMs). These proteins mediate signals to the interior of the cell via activation of heterotrimeric G proteins that in turn activate various effector proteins, ultimately resulting in a physiologic response.

Product Categories/Family for anti-GPR34 antibody

Antibodies; G Protein Receptors

References

Engemaier, E., et al. Genomics 87(2):254-264(2006). Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005). Jacobi, F.K., et al. Hum. Genet. 107(1):89-91(2000). Schoneberg, T., et al. Biochim. Biophys. Acta 1446 (1-2), 57-70 (1999): Marchese, A., et al. Genomics 56(1):12-21(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 148225857
• NCBI GeneID: 2857
• NCBI Accession #: NP_001091048.1
• NCBI GenBank Nucleotide #: NM_001097579.1
• UniProt Accession #: Q9UPC5; O95853
• Molecular Weight: 43,860 Da
• NCBI Official Full Name: probable G-protein coupled receptor 34
• NCBI Official Synonym Full Names: G protein-coupled receptor 34
• NCBI Official Symbol: GPR34
• NCBI Protein Information: probable G-protein coupled receptor 34
• UniProt Protein Name: Probable G-protein coupled receptor 34
• Protein Family: Probable G-protein coupled receptor
• UniProt Gene Name: GPR34
• UniProt Entry Name: GPR34_HUMAN

NCBI Description

G protein-coupled receptors (GPCRs), such as GPR34, are integral membrane proteins containing 7 putative transmembrane domains (TMs). These proteins mediate signals to the interior of the cell via activation of heterotrimeric G proteins that in turn activate various effector proteins, ultimately resulting in a physiologic response.[supplied by OMIM, Apr 2006]

Uniprot Description

GPR34: Orphan receptor. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: integral to plasma membrane

Molecular Function: G-protein coupled receptor activity; purinergic nucleotide receptor activity, G-protein coupled

Biological Process: G-protein coupled receptor protein signaling pathway