Rabbit GPR216 Polyclonal Antibody | anti-GPR216 antibody

GPR216 polyclonal antibody

Cat. Num. AAA3014906

• Gene Names: SESN1; PA26; SEST1
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Synonyms: GPR216; Polyclonal Antibody; GPR216 polyclonal antibody; Sestrin-1; p53-regulated protein PA26; SESN1; PA26; SEST1; anti-GPR216 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SESN1 polyclonal antibody detects endogenous levels of SESN1 protein.
• Purity/Purification: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GPR216 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1000
• Immunogen: Synthetic peptide, corresponding to Human SESN1
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -25 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of SESN1 polyclonal antibody at 1:500 dilutionLaneA: Hela whole cell lysateLaneB: PC12 whole cell lysate)

Related Product Information for anti-GPR216 antibody

Cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, presumably to allow time for DNA repair prior to entry into S and M phase, respectively. The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD 45 and the mitotic inhibitory protein p21. GADD 45 stimulates DNA excision repair in vitro and inhibits entry of cells into S phase, and it apparently acts in concert with GADD 153 in inducing growth arrest. A related DNA-damage inducible gene, GADD 34 synergizes with GADD 45 or GADD 153 in suppressing cell growth. PEG-3 (progression elevated gene-3) shares significant homology with GADD 34 and is inducible by DNA damage. An additional GADD related gene, PA26, is a possible target of p53. Three isoforms of PA26 have been identified as PA26-T1, PA26-T2 and PA26-T3.

NCBI and Uniprot Product Information

• NCBI GI #: 315709500
• NCBI GeneID: 27244
• NCBI Accession #: NP_001186862.1
• NCBI GenBank Nucleotide #: NM_001199933.1
• UniProt Accession #: Q9Y6P5; Q2M2B7; Q5T316; Q9NV00; Q9UPD5; Q9Y6P6
• Molecular Weight: 56,557 Da
• NCBI Official Full Name: sestrin-1 isoform 2
• NCBI Official Synonym Full Names: sestrin 1
• NCBI Official Symbol: SESN1
• NCBI Official Synonym Symbols: PA26; SEST1
• NCBI Protein Information: sestrin-1; p53 activated gene 26; p53 regulated PA26 nuclear protein
• UniProt Protein Name: Sestrin-1
• UniProt Gene Name: SESN1
• UniProt Synonym Gene Names: PA26; SEST1
• UniProt Entry Name: SESN1_HUMAN

NCBI Description

This gene encodes a member of the sestrin family. Sestrins are induced by the p53 tumor suppressor protein and play a role in the cellular response to DNA damage and oxidative stress. The encoded protein mediates p53 inhibition of cell growth by activating AMP-activated protein kinase, which results in the inhibition of the mammalian target of rapamycin protein. The encoded protein also plays a critical role in antioxidant defense by regenerating overoxidized peroxiredoxins, and the expression of this gene is a potential marker for exposure to radiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

SESN1: Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth. Belongs to the sestrin family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleus

Biological Process: negative regulation of cell proliferation; regulation of protein kinase B signaling cascade; cell cycle arrest; response to DNA damage stimulus