Rabbit anti-Human, Mouse PDE6D Polyclonal Antibody | anti-PDE6D antibody

PDE6D, ID (PDE6D, PDED, Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta, Protein p17) (Azide free) (HRP)

Cat. Num. AAA6332181

• Gene Names: PDE6D; PDED; JBTS22
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDE6D; Polyclonal Antibody; ID (PDE6D; PDED; Retinal rod rhodopsin-sensitive cGMP 3'; 5'-cyclic phosphodiesterase subunit delta; Protein p17) (Azide free) (HRP); Protein p17; anti-PDE6D antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PDE6D antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PDE6D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-55 amino acids from the Central region of human PDE6D.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PDE6D Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PDE6D antibody detected the PDE6D protein (arrow).)

Western Blot (WB)

(PDE6D Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the PDE6D antibody detected the PDE6D protein (arrow).)

Related Product Information for anti-PDE6D antibody

Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation.

Product Categories/Family for anti-PDE6D antibody

Antibodies; Enzymes; Phosphodiesterase

References

Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008). Wilson, S.J., et al. J. Biol. Chem. 281(17):11780-11786(2006). Stelzl, U., et al. Cell 122(6):957-968(2005). Hanzal-Bayer, M., et al. EMBO J. 21(9):2095-2106(2002). Nancy, V., et al. J. Biol. Chem. 277(17):15076-15084(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4505671
• NCBI GeneID: 5147
• NCBI Accession #: NP_002592.1
• NCBI GenBank Nucleotide #: NM_002601.3
• UniProt Accession #: O43924; O43250
• Molecular Weight: 17420
• NCBI Official Full Name: retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform 1
• NCBI Official Synonym Full Names: phosphodiesterase 6D, cGMP-specific, rod, delta
• NCBI Official Symbol: PDE6D
• NCBI Official Synonym Symbols: PDED; JBTS22
• NCBI Protein Information: retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
• UniProt Protein Name: Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
• Protein Family: Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE6D
• UniProt Synonym Gene Names: PDED; GMP-PDE delta
• UniProt Entry Name: PDE6D_HUMAN

NCBI Description

This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]

Uniprot Description

PDE6D: Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. Belongs to the PDE6D/unc-119 family.

Chromosomal Location of Human Ortholog: 2q35-q36

Cellular Component: cytoskeleton; cytoplasmic vesicle membrane; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; 3',5'-cyclic-nucleotide phosphodiesterase activity; Rab GTPase binding; GTPase inhibitor activity

Biological Process: visual perception; metabolic process; organelle organization and biogenesis; negative regulation of catalytic activity; response to stimulus

Disease: Joubert Syndrome 22