Western Blot (WB)
(Western blot (WB) analysis of QSK polyclonal antibody at 1:500 dilutionLaneA: L02 whole cell lysateLaneB: MCF-7 whole cell lysateLaneC: A549 whole cell lysateLaneD: CT-26 whole cell lysateLaneE: PC12 whole cell lysate)
Rabbit GPR211 Polyclonal Antibody | anti-GPR211 antibody
GPR211 polyclonal antibody
Gene Names
SIK3; L19; QSK; SIK-3
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
Synonyms
GPR211; Polyclonal Antibody; GPR211 polyclonal antibody; Serine/threonine-protein kinase SIK3; Salt-inducible kinase 3; SIK-3; Serine/threonine-protein kinase QSK; SIK3; KIAA0999; QSK; L19; anti-GPR211 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
QSK polyclonal antibody detects endogenous levels of QSK protein.
Purity/Purification
The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.4
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GPR211 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500~1000
Immunogen
Synthetic peptide, corresponding to Human QSK
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -25 degree C long term. Avoid freeze-thaw cycles.
Western Blot (WB)
(Western blot (WB) analysis of QSK polyclonal antibody at 1:500 dilutionLaneA: L02 whole cell lysateLaneB: MCF-7 whole cell lysateLaneC: A549 whole cell lysateLaneD: CT-26 whole cell lysateLaneE: PC12 whole cell lysate)
Western Blot (WB)
(Western blot (WB) analysis of QSK polyclonal antibody at 1:500 dilutionLaneA: L02 whole cell lysateLaneB: MCF-7 whole cell lysateLaneC: A549 whole cell lysateLaneD: CT-26 whole cell lysateLaneE: PC12 whole cell lysate)
Related Product Information for anti-GPR211 antibody
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/ threonine (Ser/Thr) protein kinases. The salt-inducible kinases (SIKs) are a family of related serine-threonine kinases and are key enzymes that modulate important processes such as steroid hormone biosynthesis and Insulin signaling in adipocytes. QSK, also known as L19 or SIK3 (salt-inducible kinase 3), is a 1,263 amino acid cytoplasmic protein belonging to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and the AMPK subfamily. Ubiquitously expressed, QSK consists of one protein kinase domain and a UBA domain. QSK is activated by 14-3-3 zeta and utilizes magnesium as a cofactor. QSK exists as three alternatively spliced isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
139,980 Da
NCBI Official Full Name
serine/threonine-protein kinase SIK3
NCBI Official Synonym Full Names
SIK family kinase 3
NCBI Official Symbol
SIK3
NCBI Official Synonym Symbols
L19; QSK; SIK-3
NCBI Protein Information
serine/threonine-protein kinase SIK3; salt-inducible kinase 3; serine/threonine-protein kinase QSK
UniProt Protein Name
Serine/threonine-protein kinase SIK3
UniProt Gene Name
SIK3
UniProt Synonym Gene Names
KIAA0999; QSK; SIK-3
UniProt Entry Name
SIK3_HUMAN
Uniprot Description
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium.
Enzyme regulation: Activated by phosphorylation on Thr-163
By similarity. Ref.1
Subunit structure: Binds to and is activated by YWHAZ when phosphorylated on Thr-163. Ref.8
Subcellular location: Cytoplasm. Note: Locates to punctate structures within the cytoplasm on binding to YWHAZ. Ref.8
Post-translational modification: Phosphorylated at Thr-163 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Ref.1
Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.Contains 1 protein kinase domain.Contains 1 UBA domain.
Sequence caution: The sequence AAT06749.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA76843.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB14006.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on GPR211
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Product Notes
The GPR211 sik3 (Catalog #AAA3014901) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR211 polyclonal antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GPR211 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500~1000. Researchers should empirically determine the suitability of the GPR211 sik3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR211, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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