Western Blot (WB)
(Western blot analysis of extracts from LOVO cells, using GPR174 antibody.)
Rabbit anti-Human, Mouse GPR174 Polyclonal Antibody | anti-GPR174 antibody
GPR174 Antibody
Gene Names
GPR174; FKSG79; GPCR17; LYPSR3
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GPR174; Polyclonal Antibody; GPR174 Antibody; FKSG79; G protein-coupled receptor 174; GP174_HUMAN; Gpr174; GRPC 174; GRPC174; jeg18; Probable G-protein coupled receptor 174; purinergic receptor fksg79; Putative purinergic receptor; Putative purinergic receptor FKSG792; anti-GPR174 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
GPR174 antibody detects endogenous levels of total GPR174
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
333
Applicable Applications for anti-GPR174 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Predicted Cross Reactivity
Horse, Rabbit, Dog
Similarity
Horse (85%), Rabbit (92%), Dog (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells, using GPR174 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells, using GPR174 antibody.)
Immunofluorescene (IF)
(MBS9606354 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9606354 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-GPR174 antibody
Function: Putative receptor for purines coupled to G-proteins.
Similarity: Belongs to the G-protein coupled receptor 1 family.
Similarity: Belongs to the G-protein coupled receptor 1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 34 kD
Predicted: 39 kDa
Predicted: 39 kDa
NCBI Official Full Name
probable G-protein coupled receptor 174
NCBI Official Synonym Full Names
G protein-coupled receptor 174
NCBI Official Symbol
GPR174
NCBI Official Synonym Symbols
FKSG79; GPCR17; LYPSR3
NCBI Protein Information
probable G-protein coupled receptor 174
UniProt Protein Name
Probable G-protein coupled receptor 174
Protein Family
UniProt Gene Name
GPR174
NCBI Description
This gene encodes a protein belonging to the G protein-coupled receptor superfamily. These proteins are characterized by the presence of seven alpha-helical transmembrane domains, and they activate or interact with various endogenous or exogenous ligands, including neurotransmitters, hormones, and odorant and taste substances. This family member is classified as an orphan receptor because the cognate ligand has not been identified. [provided by RefSeq, Sep 2011]
Uniprot Description
Putative receptor for purines coupled to G-proteins.
Research Articles on GPR174
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Product Notes
The GPR174 gpr174 (Catalog #AAA9606354) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR174 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GPR174 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the GPR174 gpr174 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR174, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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