Glucagon Like Peptide 1 (GLP1) Recombinant Protein | GLP1 recombinant protein

Recombinant Glucagon Like Peptide 1 (GLP1)

Cat. Num. AAA2105297

• Gene Names: GCG; GLP1; GLP2; GRPP; GLP-1
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Glucagon Like Peptide 1 (GLP1); Recombinant Glucagon Like Peptide 1 (GLP1); GLP1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic Expression
• Purity/Purification: > 90%
• Form/Format: Freeze-Dried Powder; PBS, pH7.4, Containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: His92~Gly128*2
• Sequence Length: 180

• Applicable Applications for GLP1 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Species: Human
• Tag: N-terminal His-Tag
• Isoelectric Point: 4.8
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Subcellular Location: Extracellular Region or Secreted
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Product Categories/Family for GLP1 recombinant protein

Endocrinology; Cardiovascular biology; Hepatology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4503945
• NCBI GeneID: 2641
• NCBI Accession #: NP_002045.1
• NCBI GenBank Nucleotide #: NM_002054.5
• UniProt Accession #: P01275
• Molecular Weight: Predicted MW: 12kDa; Accurate MW: 13kDa
• NCBI Official Full Name: glucagon preproprotein
• NCBI Official Synonym Full Names: glucagon
• NCBI Official Symbol: GCG
• NCBI Official Synonym Symbols: GLP1; GLP2; GRPP; GLP-1
• NCBI Protein Information: glucagon
• UniProt Protein Name: Glucagon
• Protein Family: Germin-like protein
• UniProt Gene Name: GCG
• UniProt Synonym Gene Names: GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2
• UniProt Entry Name: GLUC_HUMAN

NCBI Description

The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]

Uniprot Description

GCG: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. GLP-1 and GLP-2 are induced in response to nutrient ingestion. Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. Belongs to the glucagon family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q36-q37

Cellular Component: extracellular space; endoplasmic reticulum lumen; extracellular region; plasma membrane

Molecular Function: identical protein binding; protein binding; hormone activity; glucagon receptor binding; receptor binding

Biological Process: positive regulation of protein binding; positive regulation of histone H3-K4 methylation; signal transduction; positive regulation of peptidyl-serine phosphorylation; G-protein signaling, coupled to cAMP nucleotide second messenger; response to starvation; G-protein coupled receptor protein signaling pathway; cell proliferation; cellular protein metabolic process; positive regulation of protein kinase activity; positive regulation of cAMP biosynthetic process; energy reserve metabolic process; feeding behavior; regulation of insulin secretion; negative regulation of apoptosis