Rabbit anti-Human GPR132 Polyclonal Antibody | anti-GPR132 antibody

GPR132 Antibody

Cat. Num. AAA9606398

• Gene Names: GPR132; G2A
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: GPR132; Polyclonal Antibody; GPR132 Antibody; G Protein Coupled Receptor G2A; G2 accumulation protein; G2A; GP132_HUMAN; Probable G-protein coupled receptor 132; anti-GPR132 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: GPR132 antibody detects endogenous levels of total GPR132.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 380

• Applicable Applications for anti-GPR132 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000, IF/ICC: 1:100-1:500, ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human GPR132, corresponding to a region within C-terminal amino acids.
• Important: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt.

Immunofluorescence (IF)

(MBS9606398 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, using GPR132 antibody.)

Related Product Information for anti-GPR132 antibody

Function: May be a receptor for oxidized free fatty acids derived from linoleic and arachidonic acids such as 9-hydroxyoctadecadienoic acid (9-HODE). Activates a G alpha protein, most likely G alpha(q). May be involved in apoptosis. Functions at the G2/M checkpoint to delay mitosis. May function as a sensor that monitors the oxidative states and mediates appropriate cellular responses such as secretion of paracrine signals and attenuation of proliferation. May mediate ths accumulation of intracellular inositol phosphates at acidic pH through proton-sensing activity.

Similarity: Belongs to the G-protein coupled receptor 1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 524579300
• NCBI GeneID: 29933
• NCBI Accession #: NP_001265623.1
• NCBI GenBank Nucleotide #: NM_001278694.1
• UniProt Accession #: Q9UNW8; Q9BSU2; A8K7X7; B4E144
• Molecular Weight: 42 kD
• NCBI Official Full Name: probable G-protein coupled receptor 132 isoform 1
• NCBI Official Synonym Full Names: G protein-coupled receptor 132
• NCBI Official Symbol: GPR132
• NCBI Official Synonym Symbols: G2A
• NCBI Protein Information: probable G-protein coupled receptor 132
• UniProt Protein Name: Probable G-protein coupled receptor 132
• Protein Family: Probable G-protein coupled receptor
• UniProt Gene Name: GPR132
• UniProt Synonym Gene Names: G2A

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

May be a receptor for oxidized free fatty acids derived from linoleic and arachidonic acids such as 9-hydroxyoctadecadienoic acid (9-HODE). Activates a G alpha protein, most likely G alpha(q). May be involved in apoptosis. Functions at the G2/M checkpoint to delay mitosis. May function as a sensor that monitors the oxidative states and mediates appropriate cellular responses such as secretion of paracrine signals and attenuation of proliferation. May mediate ths accumulation of intracellular inositol phosphates at acidic pH through proton-sensing activity.