Typical Testing Data/Standard Curve (for reference only)
Chicken CD320 antigen (CD320) ELISA Kit | CD320 elisa kit
Chicken CD320 antigen (CD320) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CD320 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CD320 antibody and an CD320-HRP conjugate. The assay sample and buffer are incubated together with CD320-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD320 concentration since CD320 from samples and CD320-HRP conjugate compete for the anti-CD320 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD320 from the sample, fewer sites are left to bind CD320-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD320 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and is involved in B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]
Uniprot Description
CD320: Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC); also called methylmalonic aciduria due to transcobalamin receptor defect. MMATC is a metabolic disorder characterized by increased blood C3- acylcarnitine levels, elevated methylmalonate and homocysteine, and low uptake of transcobalamin-bound cobalamin, but normal conversion to adenosylcobalamin and methylcobalamin. Plasma vitamin B12 and total homocysteine are normal.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 19p13.3-p13.2
Cellular Component: membrane; endoplasmic reticulum; integral to membrane; plasma membrane; endosome membrane
Molecular Function: growth factor activity; cobalamin binding
Biological Process: vitamin metabolic process; cobalamin metabolic process; regulation of vitamin metabolic process; regulation of cell growth; water-soluble vitamin metabolic process
Disease: Methylmalonic Aciduria Due To Transcobalamin Receptor Defect
Research Articles on CD320
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Product Notes
The Chicken CD320 cd320 (Catalog #AAA7209454) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7209454 ELISA Kit recognizes Chicken CD320. It is sometimes possible for the material contained within the vial of "CD320 antigen (CD320), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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