Goat GPI Polyclonal Antibody | anti-GPI antibody

Goat Polyclonal to Human GPI

Cat. Num. AAA247282

• Gene Names: GPI; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
• Reactivity: Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Pig (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: GPI; Polyclonal Antibody; Goat Polyclonal to Human GPI; Anti-GPI Antibody (N-Terminus) IHC-plus; AMF; Autocrine motility factor; Glucose phosphate isomerase; Hexose monophosphate isomerase; Hexosephosphate isomerase; Neuroleukin; Oxoisomerase; Phosphoglucose isomerase; Phosphohexomutase; Phosphohexose isomerase; SA36; PGI; SA-36; Human GPI; anti-GPI antibody

• Host: Goat
• Reactivity: Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Pig (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human GPI.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 558

• Applicable Applications for anti-GPI antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Western Blot: Approx 60kD band observed in Human Heart lysates (calculated MW of 63.1kD according to NP_000166.2). Recommended concentration: 0.1-0.3 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide C-RERMFNGEKINYT from an internal region (near N-terminus) of human GPI (NP_000166.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset (100%); Elephant, Pig (92%).
• Immunogen Type: Synthetic peptide
• Immunogen: GPI antibody was raised against synthetic peptide C-RERMFNGEKINYT from an internal region (near N-terminus) of human GPI (NP_000166.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset (100%); Elephant, Pig (92%); Panda, Bovine, Dog, Horse, Chicken, Platypus (85%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-GPI antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-GPI antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-GPI antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(GPI antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 18201905
• NCBI GeneID: 2821
• NCBI Accession #: NP_000166.2
• NCBI GenBank Nucleotide #: NM_000175.3
• UniProt Accession #: P06744; Q9BRD3; Q9BSK5; Q9UHE6; B4DG39
• Molecular Weight: 64,325 Da
• NCBI Official Full Name: glucose-6-phosphate isomerase isoform 2
• NCBI Official Synonym Full Names: glucose-6-phosphate isomerase
• NCBI Official Symbol: GPI
• NCBI Official Synonym Symbols: AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
• NCBI Protein Information: glucose-6-phosphate isomerase; neuroleukin; oxoisomerase; sperm antigen 36; sperm antigen-36; phosphohexomutase; phosphosaccharomutase; phosphohexose isomerase; phosphoglucose isomerase; autocrine motility factor; hexosephosphate isomerase; glucose phosphate isomerase; hexose monophosphate isomerase
• UniProt Protein Name: Glucose-6-phosphate isomerase
• Protein Family: GPI-anchored protein
• UniProt Gene Name: GPI
• UniProt Synonym Gene Names: GPI; AMF; NLK; PGI; PHI; SA-36
• UniProt Entry Name: G6PI_HUMAN

NCBI Description

This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - pentose phosphate pathway; Cytokine; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Isomerase; EC 5.3.1.9; Apoptosis

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: nucleoplasm; extracellular space; neuron projection; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: monosaccharide binding; glucose-6-phosphate isomerase activity; growth factor activity; cytokine activity; intramolecular transferase activity

Biological Process: aldehyde catabolic process; methylglyoxal biosynthetic process; learning and/or memory; glycolysis; hemostasis; carbohydrate metabolic process; glucose 6-phosphate metabolic process; glucose metabolic process; pathogenesis; negative regulation of neuron apoptosis; negative regulation of caspase activity; angiogenesis; humoral immune response; gluconeogenesis

Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency