Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat Pleiotrophin Polyclonal Antibody | anti-PTN antibody
Goat anti-Pleiotrophin, Biotinylated Antibody
Western Blot: Approx 18kDa band observed in Mouse fetal Brain lysates (calculated MW of 18.9kDa according to Mouse NP_032999.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
Uniprot Description
PTN: Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti- apoptotic signaling of PTN and regulation of cell proliferation. Belongs to the pleiotrophin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 7q33
Cellular Component: basement membrane; cell surface; endoplasmic reticulum; extracellular space; membrane; neuromuscular junction; perinuclear region of cytoplasm
Molecular Function: growth factor activity; heparin binding; protein phosphatase inhibitor activity
Biological Process: bone mineralization; cerebellum development; endothelial cell differentiation; heart development; learning; liver development; lung development; negative regulation of angiogenesis; negative regulation of catalytic activity; negative regulation of cell migration; negative regulation of epithelial cell proliferation; negative regulation of membrane potential; nervous system development; positive regulation of apoptosis; positive regulation of cell division; positive regulation of cell proliferation; regulation of cell shape; response to activity; response to drug; response to estradiol stimulus; response to progesterone stimulus; spinal cord development; thalamus development; transmembrane receptor protein tyrosine phosphatase signaling pathway
Research Articles on PTN
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Product Notes
The PTN ptn (Catalog #AAA423654) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Pleiotrophin, Biotinylated Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Pleiotrophin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 18kDa band observed in Mouse fetal Brain lysates (calculated MW of 18.9kDa according to Mouse NP_032999.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the PTN ptn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KEGKKQEKML D. It is sometimes possible for the material contained within the vial of "Pleiotrophin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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