Rabbit anti-Human GPI Polyclonal Antibody | anti-GPI antibody
Anti-GPI Antibody IHC-plus
IHC-P (10 ug/ml)
WB (1:5000 - 1:20000)
Immunohistochemistry - Paraffin (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry - Paraffin (IHC)
(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(GPI antibody detects GPI protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: GPI protein stained by Anti-GPI antibody diluted at 1:500. Blue: Hoechst 33342 staining.)
Western Blot (WB)
(Sample (30 ug of whole cell lysate). A: H1299. B: Raji. 7.5% SDS PAGE. GPI antibody diluted at 1:10000.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Uniprot Description
G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Protein type: Isomerase; Carbohydrate Metabolism - pentose phosphate pathway; Apoptosis; EC 5.3.1.9; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Cytokine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - starch and sucrose
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: cytoplasm; cytosol; extracellular space; membrane; neuron projection; nucleoplasm; plasma membrane
Molecular Function: cytokine activity; glucose-6-phosphate isomerase activity; growth factor activity; intramolecular transferase activity; monosaccharide binding; ubiquitin protein ligase binding
Biological Process: aldehyde catabolic process; angiogenesis; carbohydrate metabolic process; gene expression; gluconeogenesis; glucose 6-phosphate metabolic process; glucose metabolic process; hemostasis; humoral immune response; learning and/or memory; methylglyoxal biosynthetic process; negative regulation of caspase activity; negative regulation of neuron apoptosis; transcription initiation from RNA polymerase II promoter
Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency
