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Western Blot (WB) (GP2 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the GP2 antibody detected the GP2 protein (arrow).)

Rabbit anti-Human GP2 Polyclonal Antibody | anti-GP2 antibody

GP2, NT (GP2, Pancreatic secretory granule membrane major glycoprotein GP2, Pancreatic zymogen granule membrane protein GP-2, ZAP75) (PE)

Gene Names
GP2; ZAP75
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GP2; Polyclonal Antibody; NT (GP2; Pancreatic secretory granule membrane major glycoprotein GP2; Pancreatic zymogen granule membrane protein GP-2; ZAP75) (PE); ZAP75; anti-GP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-GP2 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
GP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-85 amino acids from the N-terminal region of human GP2.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GP2 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the GP2 antibody detected the GP2 protein (arrow).)

Western Blot (WB) (GP2 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the GP2 antibody detected the GP2 protein (arrow).)

Immunohistochemistry (IHC)

(GP2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (GP2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-GP2 antibody
The specific function of this protein remains unknown.
Product Categories/Family for anti-GP2 antibody
References
Kottgen, A., et al. Nat. Genet. 42(5):376-384(2010). Masson, E., et al. Pancreas 39(3):353-358(2010). Witt, H., et al. Pancreas 39(2):188-192(2010). Boulling, A., et al. Mol. Genet. Metab. 99(3):319-324(2010). Hase, K., et al. Nature 462(7270):226-230(2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,039 Da
NCBI Official Full Name
pancreatic secretory granule membrane major glycoprotein GP2 isoform 1 preproprotein
NCBI Official Synonym Full Names
glycoprotein 2 (zymogen granule membrane)
NCBI Official Symbol
GP2
NCBI Official Synonym Symbols
ZAP75
NCBI Protein Information
pancreatic secretory granule membrane major glycoprotein GP2; pancreatic zymogen granule membrane associated protein GP2
UniProt Protein Name
Pancreatic secretory granule membrane major glycoprotein GP2
Protein Family
UniProt Gene Name
GP2
UniProt Entry Name
GP2_HUMAN

NCBI Description

This gene encodes an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. The encoded protein binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

GP2: 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: apical plasma membrane

Molecular Function: antigen binding

Biological Process: antigen transcytosis by M cells in mucosal-associated lymphoid tissue

Research Articles on GP2

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Product Notes

The GP2 gp2 (Catalog #AAA6303391) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GP2, NT (GP2, Pancreatic secretory granule membrane major glycoprotein GP2, Pancreatic zymogen granule membrane protein GP-2, ZAP75) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GP2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GP2 gp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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