Monkey Protein Kinase C Iota ELISA Kit | PKCi elisa kit

Monkey Protein Kinase C Iota ELISA Kit

Cat. Num. AAA744607

• Gene Names: PRKCI; PKCI; DXS1179E; nPKC-iota
• Reactivity: Monkey
• Synonyms: Protein Kinase C Iota; Monkey Protein Kinase C Iota ELISA Kit; PKCi elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PKCi ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey PKCi. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This PKCi ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey PKCi. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKCi elisa kit

Principle of the Assay: PKCi ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKCi antibody and an PKCi-HRP conjugate. The assay sample and buffer are incubated together with PKCi-HRP conjugate in pre-coated plate for one hour.After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKCi concentration since PKCi from samples and PKCi-HRP conjugate compete for the anti-PKCi antibody binding site. Since the number of sites is limited, as more sites are occupied by PKCi from the sample, fewer sites are left to bind PKCi-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKCi concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PKCi elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 598225
• NCBI GeneID: 5584
• Molecular Weight: 68,262 Da
• NCBI Official Full Name: protein kinase C iota
• NCBI Official Synonym Full Names: protein kinase C, iota
• NCBI Official Symbol: PRKCI
• NCBI Official Synonym Symbols: PKCI; DXS1179E; nPKC-iota
• NCBI Protein Information: protein kinase C iota type; PRKC-lambda/iota; aPKC-lambda/iota; atypical protein kinase C-lambda/iota
• UniProt Protein Name: Protein kinase C iota type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCI
• UniProt Synonym Gene Names: DXS1179E; PRKC-lambda/iota; aPKC-lambda/iota
• UniProt Entry Name: KPCI_HUMAN

NCBI Description

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCI: an AGC protein kinase of the PKC family. A calcium-independent, phospholipid-dependent atypical protein kinase C. Is not activated by phorbolesters or diaglycerol. May play role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets.

Protein type: Kinase, protein; Motility/polarity/chemotaxis; Oncoprotein; Cell development/differentiation; Protein kinase, AGC; EC 2.7.11.13; Cell adhesion; Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Iota subfamily

Chromosomal Location of Human Ortholog: 3q26.3

Cellular Component: Golgi membrane; microtubule cytoskeleton; intercellular bridge; protein complex; tight junction; leading edge; cytoplasm; apical plasma membrane; plasma membrane; cytosol; nucleus; endosome

Molecular Function: protein serine/threonine kinase activity; protein domain specific binding; protein binding; protein kinase C activity; metal ion binding; phospholipid binding; ATP binding; protein kinase activity

Biological Process: intercellular junction assembly and maintenance; cell migration; secretion; nerve growth factor receptor signaling pathway; establishment of apical/basal cell polarity; actin filament organization; cytoskeleton organization and biogenesis; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; establishment and/or maintenance of epithelial cell polarity; vesicle-mediated transport; positive regulation of glucose import; eye photoreceptor cell development; cellular response to insulin stimulus; Golgi vesicle budding; negative regulation of neuron apoptosis; protein targeting to membrane; negative regulation of apoptosis