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Western Blot (WB)
(Anti-GOT2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HL60 Whole Cell Lysate Lane D: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:48 kDa Observed band size:43 kDa)
Rabbit GOT2 Polyclonal Antibody | anti-GOT2 antibody
Anti-GOT2 Antibody, Rabbit Polyclonal
Gene Names
GOT2; KAT4; KATIV; mitAAT
Reactivity
Human, Mouse, RatPredicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
GOT2; Polyclonal Antibody; Anti-GOT2 Antibody; Rabbit Polyclonal; GOT2 Antibody; Rabbit PAb; Antigen Affinity Purified; glutamic-oxaloacetic transaminase 2; mitochondrial; Anti-KAT4 Antibody; Anti-KATIV Antibody; Anti-mitAAT Antibody; anti-GOT2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology)
Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GOT2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GOT2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:500-1:2000
IHC-P: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the center region of the Human GOT2
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human GOT2, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-GOT2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HL60 Whole Cell Lysate Lane D: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:48 kDa Observed band size:43 kDa)
Western Blot (WB)
(Anti-GOT2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HL60 Whole Cell Lysate Lane D: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:48 kDa Observed band size:43 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining GOT2 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining GOT2 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining GOT2 in human hepatoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining GOT2 in human hepatoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining GOT2 in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining GOT2 in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining GOT2 in rat liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining GOT2 in rat liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-GOT2 antibody
GOT2 (Glutamic-Oxaloacetic Transaminase 2) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. The encoded protein is a mitochondrial enzyme that oxidizes glutamate to produce alpha-ketoglutarate for the Krebs cycle, eventually leading to the production of adenosine triphosphate (ATP). GOT2 belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. GOT2-mediated ATP synthesis may be a pivotal mechanism underlying the protective effect of febuxostat against neurodegeneration in experimental autoimmune encephalomyelitis (EAE). The high aberrant GOT2 expression is prognostic in diffuse large B-cell lymphoma. GOT2 is broadly expressed in the heart, liver, and other tissues. Diseases associated with GOT2 include Epileptic Encephalopathy, Early Infantile, 82, and Acute Vascular Insufficiency Of Intestine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
430
NCBI Official Full Name
aspartate aminotransferase, mitochondrial
NCBI Official Synonym Full Names
glutamic-oxaloacetic transaminase 2, mitochondrial
NCBI Official Symbol
GOT2
NCBI Official Synonym Symbols
KAT4; KATIV; mitAAT
NCBI Protein Information
aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; mAspAT; transaminase A; aspartate transaminase 2; fatty acid-binding protein; aspartate aminotransferase 2; kynurenine aminotransferase 4; kynurenine aminotransferase IV; glutamate oxaloacetate tr
UniProt Protein Name
Aspartate aminotransferase, mitochondrial
UniProt Gene Name
GOT2
UniProt Synonym Gene Names
mAspAT; FABP-1; FABPpm
UniProt Entry Name
AATM_HUMAN
Similar Products
Product Notes
The GOT2 got2 (Catalog #AAA8102866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GOT2 Antibody, Rabbit Polyclonal reacts with Human, Mouse, Rat Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's GOT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:500-1:2000. Researchers should empirically determine the suitability of the GOT2 got2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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