Mouse TLN1 Monoclonal Antibody | anti-TLN1 antibody

TLN1 (Talin 1, ILWEQ, KIAA1027, TLN) (FITC)

Cat. Num. AAA6175479

• Gene Names: TLN1; TLN; ILWEQ
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: TLN1; Monoclonal Antibody; TLN1 (Talin 1; ILWEQ; KIAA1027; TLN) (FITC); Talin 1; TLN; anti-TLN1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3,k
• Clone Number: 5C1
• Specificity: Recognizes TLN1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-TLN1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: TLN1 (NP_006280, 1052aa-1149aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Immunoperoxidase of monoclonal antibody to TLN1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to TLN1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])

Western Blot (WB)

(TLN1 monoclonal antibody (M05), clone 5C1. Western Blot analysis of TLN1 expression in Raw 264.7.)

Testing Data

(Detection limit for recombinant GST tagged TLN1 is approximately 0.03ng/ml as a capture antibody.)

Western Blot (WB)

(TLN1 monoclonal antibody (M05), clone 5C1. Western Blot analysis of TLN1 expression in PC-12 (Cat # L012V1).)

Related Product Information for anti-TLN1 antibody

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq]

Product Categories/Family for anti-TLN1 antibody

Antibodies; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 223029410
• NCBI GeneID: 7094
• NCBI Accession #: NP_006280
• NCBI GenBank Nucleotide #: NM_006289
• UniProt Accession #: Q9Y490; Q86YD0; Q9NZQ2; Q9UHH8; Q9UPX3; A6NMY0
• Molecular Weight: 269,767 Da
• NCBI Official Full Name: talin-1
• NCBI Official Synonym Full Names: talin 1
• NCBI Official Symbol: TLN1
• NCBI Official Synonym Symbols: TLN; ILWEQ
• NCBI Protein Information: talin-1
• UniProt Protein Name: Talin-1
• Protein Family: Talin
• UniProt Gene Name: TLN1
• UniProt Synonym Gene Names: KIAA1027; TLN
• UniProt Entry Name: TLN1_HUMAN

Uniprot Description

Talin-1: a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: ruffle; cell surface; focal adhesion; cytoplasm; plasma membrane; extracellular region; microtubule organizing center; intercellular junction; cytosol

Molecular Function: integrin binding; actin filament binding; LIM domain binding; protein binding; structural constituent of cytoskeleton; insulin receptor binding; vinculin binding

Biological Process: intercellular junction assembly; axon guidance; platelet activation; unfolded protein response; cytoskeletal anchoring; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; platelet degranulation; muscle contraction; cell-substrate junction assembly; cortical actin cytoskeleton organization and biogenesis; cell motility; blood coagulation