Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells using TUBGCP5 antibody.)
Rabbit TUBGCP5 Polyclonal Antibody | anti-TUBGCP5 antibody
TUBGCP5 Antibody
Gene Names
TUBGCP5; GCP5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TUBGCP5; Polyclonal Antibody; TUBGCP5 Antibody; Gamma tubulin complex component GCP5; Gamma-tubulin complex component 5; GCP 5; GCP-5; GCP5; GCP5_HUMAN; KIAA1899; OTTHUMP00000159254; Tubgcp5; Tubulin gamma complex associated protein 5; anti-TUBGCP5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TUBGCP5 antibody detects endogenous levels of total TUBGCP5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1024
Applicable Applications for anti-TUBGCP5 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm > Cytoskeleton > Centrosome.
Tissue Specificity
Widely expressed, with highest levels in heart and skeletal muscle and moderate levels in brain.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells using TUBGCP5 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells using TUBGCP5 antibody.)
Immunofluorescene (IF)
(MBS9605531 staining HT29 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9605531 staining HT29 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-TUBGCP5 antibody
Function: Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
Subunit Structure: Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6.
Similarity: Belongs to the TUBGCP family.
Subunit Structure: Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6.
Similarity: Belongs to the TUBGCP family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 118 kD
Predicted: 119 kDa
Predicted: 119 kDa
NCBI Official Full Name
gamma-tubulin complex component 5 isoform b
NCBI Official Synonym Full Names
tubulin gamma complex associated protein 5
NCBI Official Symbol
TUBGCP5
NCBI Official Synonym Symbols
GCP5
NCBI Protein Information
gamma-tubulin complex component 5
UniProt Protein Name
Gamma-tubulin complex component 5
Protein Family
UniProt Gene Name
TUBGCP5
UniProt Synonym Gene Names
GCP5; KIAA1899; GCP-5
Uniprot Description
Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
Research Articles on TUBGCP5
Similar Products
Product Notes
The TUBGCP5 tubgcp5 (Catalog #AAA9605531) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TUBGCP5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TUBGCP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the TUBGCP5 tubgcp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUBGCP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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