Western Blot (WB)
(JARID1B Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the JARID1B antibody detected the JARID1B protein (arrow).)
Rabbit anti-Mouse JARID1B Polyclonal Antibody | anti-JARID1B antibody
JARID1B, NT (KDM5B, JARID1B, PLU1, RBBP2H1, Lysine-specific demethylase 5B, Cancer/testis antigen 31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1) (Azide free) (HRP)
Gene Names
KDM5B; CT31; PLU1; PUT1; PLU-1; JARID1B; PPP1R98; RBBP2H1A
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
JARID1B; Polyclonal Antibody; NT (KDM5B; PLU1; RBBP2H1; Lysine-specific demethylase 5B; Cancer/testis antigen 31; Histone demethylase JARID1B; Jumonji/ARID domain-containing protein 1B; PLU-1; Retinoblastoma-binding protein 2 homolog 1) (Azide free) (HRP); KDM5B; Retinoblastoma-binding protein 2 homolog 1; anti-JARID1B antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-JARID1B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
JARID1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-225 amino acids from the N-terminal region of human JARID1B.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(JARID1B Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the JARID1B antibody detected the JARID1B protein (arrow).)
Western Blot (WB)
(JARID1B Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the JARID1B antibody detected the JARID1B protein (arrow).)
Related Product Information for anti-JARID1B antibody
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
Product Categories/Family for anti-JARID1B antibody
References
Kim, J., et al. Biochem. Biophys. Res. Commun. 401(3):412-416(2010). Krishnakumar, R., et al. Mol. Cell 39(5):736-749(2010). Yao, W., et al. Biochem. Biophys. Res. Commun. 396(2):323-328(2010). Roesch, A., et al. Cell 141(4):583-594(2010). Hayami, S., et al. Mol. Cancer 9, 59 (2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
179,409 Da
NCBI Official Full Name
Homo sapiens lysine (K)-specific demethylase 5B (KDM5B), mRNA
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 5B
NCBI Official Symbol
KDM5B
NCBI Official Synonym Symbols
CT31; PLU1; PUT1; PLU-1; JARID1B; PPP1R98; RBBP2H1A
NCBI Protein Information
lysine-specific demethylase 5B; RBP2-H1; cancer/testis antigen 31; histone demethylase JARID1B; jumonji, AT rich interactive domain 1B; jumonji/ARID domain-containing protein 1B; protein phosphatase 1, regulatory subunit 98; putative DNA/chromatin binding
UniProt Protein Name
Lysine-specific demethylase 5B
UniProt Gene Name
KDM5B
UniProt Synonym Gene Names
JARID1B; PLU1; RBBP2H1; CT31; RBP2-H1
Uniprot Description
JARID1B: Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. Interacts with FOXG1B, PAX9, MYC, MYCN and RB1. Interacts with HDAC1, HDAC4, HDAC5 and HDAC7. Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer. Belongs to the JARID1 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cancer Testis Antigen (CTA); Demethylase; EC 1.14.11.-; Oxidoreductase; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: histone demethylase activity; histone demethylase activity (H3-K4 specific); protein binding; transcription corepressor activity; transcription factor activity
Research Articles on JARID1B
Similar Products
Product Notes
The JARID1B kdm5b (Catalog #AAA6311569) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JARID1B, NT (KDM5B, JARID1B, PLU1, RBBP2H1, Lysine-specific demethylase 5B, Cancer/testis antigen 31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1) (Azide free) (HRP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's JARID1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the JARID1B kdm5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JARID1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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