Rabbit anti-Human GOLPH2/GOLM1 Polyclonal Antibody | anti-GOLM1 antibody

Anti-GOLPH2/GOLM1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106687

• Gene Names: GOLM1; GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: GOLPH2/GOLM1; Polyclonal Antibody; Anti-GOLPH2/GOLM1 Antibody; Rabbit Polyclonal; GOLM1/GP73 Antibody; Rabbit PAb; Antigen Affinity Purified; golgi membrane protein 1; Anti-bA379P1.3 Antibody; Anti-C9orf155 Antibody; Anti-GOLPH2 Antibody; Anti-GP73 Antibody; Anti-HEL46 Antibody; Anti-PSEC0257 Antibody; anti-GOLM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GOLM1/GP73
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GOLM1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:2500-1:10,000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human GOLM1/GP73 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human GOLM1/GP73 (rh GOLM1/GP73). GOLM1/GP73 specific IgG was purified by Human GOLM1/GP73 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human GP73 in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human GP73 in human hepatoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(GP73 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-GP73 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GP73 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 45 kDaObserved band size: 70 kDa)

Western Blot (WB)

(Anti-GP73 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Hela Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:45 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.))

Immunofluorescence (IF)

(Immunofluorescence staining of GP73 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human GP73 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Western Blot (WB)

(Anti-GP73 rabbit polyclonal antibody at 1:500 dilution Lane A: GOLM1 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:45 kDa Observed band size: kDa)

Related Product Information for anti-GOLM1 antibody

Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2, and GOLM1, is a protein that belongs to the GOLM1/CASC4 family. GOLM1 is widely expressed. It is highly expressed in the colon, prostate, trachea, and stomach. It is expressed at a lower level in testis, muscle, lymphoid tissues, white blood cells, and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at a low level in the normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncytial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.

References

Kladney R.D., et al., (2000), GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 249:53-65.Clark H.F., et al., (2003), The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.Genome Res. 13:2265-2270.Puri S., et al., (2002), Cycling of early Golgi proteins via the cell surface and endosomes upon lumenal pH disruption.Traffic 3:641-653.

NCBI and Uniprot Product Information

• NCBI GI #: 51316027
• NCBI GeneID: 51280
• UniProt Accession #: Q8NBJ4; Q6IAF4; Q9NRB9
• Molecular Weight: 401
• NCBI Official Full Name: Golgi membrane protein 1
• NCBI Official Synonym Full Names: golgi membrane protein 1
• NCBI Official Symbol: GOLM1
• NCBI Official Synonym Symbols: GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
• NCBI Protein Information: Golgi membrane protein 1; golgi protein, 73-kD; golgi phosphoprotein 2; golgi membrane protein GP73; epididymis luminal protein 46
• UniProt Protein Name: Golgi membrane protein 1
• Protein Family: Golgi membrane protein
• UniProt Gene Name: GOLM1
• UniProt Synonym Gene Names: C9orf155; GOLPH2
• UniProt Entry Name: GOLM1_HUMAN

NCBI Description

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

GOLM1: Unknown. Cellular response protein to viral infection. Belongs to the GOLM1/CASC4 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q21.33

Cellular Component: Golgi apparatus; extracellular space; integral to plasma membrane

Molecular Function: protein binding

Biological Process: regulation of lipid metabolic process; nuclear organization and biogenesis