GOLPH2/GOLM1 Recombinant Protein | GOLM1 recombinant protein

GOLPH2/GOLM1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121257

• Gene Names: GOLM1; GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
• Purity: >90% as determined by SDS-PAGE
• Synonyms: GOLPH2/GOLM1; GOLPH2/GOLM1 Protein; Human; Recombinant (His Tag); Human GOLM1/GP73 Protein (His Tag); bA379P1.3 Protein; C9orf155 Protein; GOLPH2 Protein; GP73 Protein; HEL46 Protein; PSEC0257 Protein; golgi membrane protein 1; GOLM1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Val40-Leu401

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human GOLM1 isoform 1 (Q8NBJ4-1)(Val 40-Leu 401) was fused with a polyhistidine tag at the C-terminus and a Met at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GOLM1 recombinant protein

Background: Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2, and GOLM1, is a protein that belongs to the GOLM1/CASC4 family. GOLM1 is widely expressed. It is highly expressed in the colon, prostate, trachea, and stomach. It is expressed at a lower level in testis, muscle, lymphoid tissues, white blood cells, and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at a low level in the normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncytial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.

References

Kladney R.D., et al.,(2000), GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 249:53-65.Clark H.F., et al., (2003), The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.Genome Res. 13:2265-2270.Puri S., et al.,(2002), Cycling of early Golgi proteins via the cell surface and endosomes upon lumenal pH disruption.Traffic 3:641-653.

NCBI and Uniprot Product Information

• NCBI GI #: 51316027
• NCBI GeneID: 51280
• UniProt Accession #: Q8NBJ4; Q6IAF4; Q9NRB9
• Molecular Weight: 401
• NCBI Official Full Name: Golgi membrane protein 1
• NCBI Official Synonym Full Names: golgi membrane protein 1
• NCBI Official Symbol: GOLM1
• NCBI Official Synonym Symbols: GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
• NCBI Protein Information: Golgi membrane protein 1; golgi protein, 73-kD; golgi phosphoprotein 2; golgi membrane protein GP73; epididymis luminal protein 46
• UniProt Protein Name: Golgi membrane protein 1
• Protein Family: Golgi membrane protein
• UniProt Gene Name: GOLM1
• UniProt Synonym Gene Names: C9orf155; GOLPH2
• UniProt Entry Name: GOLM1_HUMAN

NCBI Description

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

GOLM1: Unknown. Cellular response protein to viral infection. Belongs to the GOLM1/CASC4 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q21.33

Cellular Component: Golgi apparatus; extracellular space; integral to plasma membrane

Molecular Function: protein binding

Biological Process: regulation of lipid metabolic process; nuclear organization and biogenesis