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Immunohistochemistry (IHC) (Immunochemical staining of human GOLM1 in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of hepatocyte and interlobular bile duct. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Rabbit anti-Human GOLPH2/GOLM1 Polyclonal Antibody | anti-GOLM1 antibody

Anti-GOLPH2/GOLM1 Antibody, Rabbit Polyclonal

Gene Names
GOLM1; GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
GOLPH2/GOLM1; Polyclonal Antibody; Anti-GOLPH2/GOLM1 Antibody; Rabbit Polyclonal; GOLM1/GP73 Antibody; Rabbit PAb; Antigen Affinity Purified; golgi membrane protein 1; Anti-bA379P1.3 Antibody; Anti-C9orf155 Antibody; Anti-GOLPH2 Antibody; Anti-GP73 Antibody; Anti-HEL46 Antibody; Anti-PSEC0257 Antibody; anti-GOLM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GOLM1/GP73
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GOLM1 antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:2500-1:10,000
Immunogen
Recombinant Human GOLM1/GP73 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human GOLM1/GP73 (rh GOLM1/GP73). GOLM1/GP73 specific IgG was purified by Human GOLM1/GP73 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human GOLM1 in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of hepatocyte and interlobular bile duct. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Immunohistochemistry (IHC) (Immunochemical staining of human GOLM1 in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of hepatocyte and interlobular bile duct. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Immunohistochemistry (IHC)

(Immunochemical staining of human GOLM1 in human endometrium with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of uterine gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Immunohistochemistry (IHC) (Immunochemical staining of human GOLM1 in human endometrium with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of uterine gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Related Product Information for anti-GOLM1 antibody
Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2, and GOLM1, is a protein that belongs to the GOLM1/CASC4 family. GOLM1 is widely expressed. It is highly expressed in the colon, prostate, trachea, and stomach. It is expressed at a lower level in testis, muscle, lymphoid tissues, white blood cells, and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at a low level in the normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncytial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.
References
Kladney R.D., et al., (2000), GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 249:53-65.Clark H.F., et al., (2003), The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.Genome Res. 13:2265-2270.Puri S., et al., (2002), Cycling of early Golgi proteins via the cell surface and endosomes upon lumenal pH disruption.Traffic 3:641-653.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
401
NCBI Official Full Name
Golgi membrane protein 1
NCBI Official Synonym Full Names
golgi membrane protein 1
NCBI Official Symbol
GOLM1
NCBI Official Synonym Symbols
GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
NCBI Protein Information
Golgi membrane protein 1; golgi protein, 73-kD; golgi phosphoprotein 2; golgi membrane protein GP73; epididymis luminal protein 46
UniProt Protein Name
Golgi membrane protein 1
Protein Family
UniProt Gene Name
GOLM1
UniProt Synonym Gene Names
C9orf155; GOLPH2
UniProt Entry Name
GOLM1_HUMAN

NCBI Description

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

GOLM1: Unknown. Cellular response protein to viral infection. Belongs to the GOLM1/CASC4 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q21.33

Cellular Component: Golgi apparatus; extracellular space; integral to plasma membrane

Molecular Function: protein binding

Biological Process: regulation of lipid metabolic process; nuclear organization and biogenesis

Research Articles on GOLM1

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Product Notes

The GOLM1 golm1 (Catalog #AAA8106688) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GOLPH2/GOLM1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GOLPH2/GOLM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:2500-1:10,000. Researchers should empirically determine the suitability of the GOLM1 golm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOLPH2/GOLM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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