Goat anti Human IgG (heavy and light chains) Secondary Antibody
Goat anti Human IgG (heavy and light chains)
Reactivity
Human
Applications
Precipitation assays
Synonyms
Goat anti Human IgG (heavy and light chains); Polyclonal Secondary Antibody; Goat anti Human IgG (heavy and light chains) secondary antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Specificity
Precipitating polyclonal Goat antiSerum to Human IgG, heavy and light chains
Applicable Applications for Goat anti Human IgG (heavy and light chains) secondary antibody
Precipitation assays
Application Notes
Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter centre well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Source
Purified normal IgG isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Product
Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added. It is reconstituted by adding 1 ml sterile distilled water.
Reactivity Note
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. This antiSerum has been tested for cross-reactivity in double radial immunodiffusion with the following results: Bovine - Dog + Horse +/- pigeon - Sheep - Cat + Guinea Pig + Monkey ++ Rabbit + Swine + Chicken - Hamster +/- Mouse +/- Rat + Turkey -
Preparation and Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Related Product Information for Goat anti Human IgG (heavy and light chains) secondary antibody
The reactivity of the antiserum is directed to the Fc and Fab subunits of IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against human serum a single characteristic precipitin line with a spur is usually obtained. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal human serum; to prepare an immunoadsorbent for the production of Ig-free human serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.
Product Categories/Family for Goat anti Human IgG (heavy and light chains) secondary antibody
Similar Products
Product Notes
The Goat anti Human IgG (heavy and light chains) (Catalog #AAA571163) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Human IgG (heavy and light chains) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Goat anti Human IgG (heavy and light chains) can be used in a range of immunoassay formats including, but not limited to, Precipitation assays. Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter centre well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells. Researchers should empirically determine the suitability of the Goat anti Human IgG (heavy and light chains) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat anti Human IgG (heavy and light chains), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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