Human MAML1 (Mastermind-like protein 1) ELISA Kit | MAML1 elisa kit

Human MAML1 (Mastermind-like protein 1) ELISA Kit

Cat. Num. AAA7608232

• Gene Names: MAML1; Mam1; Mam-1
• Reactivity: Human
• Synonyms: MAML1 (Mastermind-like protein 1); Human MAML1 (Mastermind-like protein 1) ELISA Kit; KIAA0200; Mam 1; Mam1; MAML1; mastermind like 1; Drosophila; Mastermind like protein 1; MAML1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MAML1. No significant cross-reactivity or interference between MAML1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAML1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7661990
• NCBI GeneID: 9794
• NCBI Accession #: NP_055572.1
• NCBI GenBank Nucleotide #: NM_014757.4
• UniProt Accession #: Q92585; Q9NZ12
• Molecular Weight: 108,054 Da
• NCBI Official Full Name: mastermind-like protein 1
• NCBI Official Synonym Full Names: mastermind-like 1 (Drosophila)
• NCBI Official Symbol: MAML1
• NCBI Official Synonym Symbols: Mam1; Mam-1
• NCBI Protein Information: mastermind-like protein 1; MAML1; mastermind homolog
• UniProt Protein Name: Mastermind-like protein 1
• Protein Family: Mastermind-like protein
• UniProt Gene Name: MAML1
• UniProt Synonym Gene Names: Mam-1
• UniProt Entry Name: MAML1_HUMAN

NCBI Description

This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

MAML1: Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. Interacts (via N-terminus) with NOTCH1, NOTCH2, NOTCH3 and NOTCH4 (via ankyrin repeat region). Interacts (via N-terminus) with p53 (via DNA-binding region). Forms a DNA-binding complex with Notch proteins and RBPSUH/RBP-J kappa/CBF1. Also binds CREBBP/CBP and CDK8. Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen. Belongs to the mastermind family.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nuclear speck; nucleus

Molecular Function: protein binding; peptide antigen binding; transcription coactivator activity; protein kinase binding

Biological Process: myoblast differentiation; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; activation of Notch receptor target transcription factor