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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human Trinucleotide Repeat Containing Protein 6A Polyclonal Antibody | anti-TNRC6A antibody

Trinucleotide Repeat Containing Protein 6A (TNRC6A) Polyclonal Antibody

Gene Names
TNRC6A; GW1; GW182; TNRC6; CAGH26
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Trinucleotide Repeat Containing Protein 6A; Polyclonal Antibody; Trinucleotide Repeat Containing Protein 6A (TNRC6A) Polyclonal Antibody; CAGH26; GW1; GW182; TNRC6; CAG repeat protein 26; EMSY interactor protein; GW182 autoantigen; Glycine-tryptophan protein of 182 kDa; anti-TNRC6A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TNRC6A. It has been selected for its ability to recognize TNRC6A in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
1962
Applicable Applications for anti-TNRC6A antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Fragment
TNRC6A (Thr1670~Met1962)
Quality Control
Content: The quality control contains recombinant TNRC6A (Thr1670~Met1962) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2068285
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
205,151 Da
NCBI Official Full Name
trinucleotide repeat-containing gene 6A protein isoform 2
NCBI Official Synonym Full Names
trinucleotide repeat containing 6A
NCBI Official Symbol
TNRC6A
NCBI Official Synonym Symbols
GW1; GW182; TNRC6; CAGH26
NCBI Protein Information
trinucleotide repeat-containing gene 6A protein
UniProt Protein Name
Trinucleotide repeat-containing gene 6A protein
UniProt Gene Name
TNRC6A
UniProt Synonym Gene Names
CAGH26; KIAA1460; TNRC6; Protein GW1

NCBI Description

This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. [provided by RefSeq, Jul 2008]

Uniprot Description

TNRC6A: Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA- dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. Belongs to the GW182 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: cytosol; Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm

Molecular Function: protein binding; RNA binding

Biological Process: miRNA mediated inhibition of translation; miRNA-mediated gene silencing; phosphoinositide-mediated signaling; regulation of megakaryocyte differentiation; RNA-mediated posttranscriptional gene silencing; Wnt receptor signaling pathway, calcium modulating pathway

Research Articles on TNRC6A

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Product Notes

The TNRC6A tnrc6a (Catalog #AAA2032900) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Trinucleotide Repeat Containing Protein 6A (TNRC6A) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Trinucleotide Repeat Containing Protein 6A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TNRC6A tnrc6a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Trinucleotide Repeat Containing Protein 6A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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