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Western Blot (WB) (Western blot analysis of extracts from mouse brain, using GNRHR2 Antibody.)

Rabbit anti-Human, Mouse GNRHR2 Polyclonal Antibody | anti-GNRHR2 antibody

GNRHR2 Antibody

Gene Names
GNRHR2; GnRH-II-R
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GNRHR2; Polyclonal Antibody; GNRHR2 Antibody; anti-GNRHR2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
GNRHR2 antibody detects endogenous levels of total GNRHR2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
292
Applicable Applications for anti-GNRHR2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Plasma Membrane
Tissue Specificity
Expressed in many tissues.
Predicted Cross Reactivity
Pig
Similarity
Pig (82%)
Conjugation
Unconjugated

Western Blot (WB)

(Western blot analysis of extracts from mouse brain, using GNRHR2 Antibody.)

Western Blot (WB) (Western blot analysis of extracts from mouse brain, using GNRHR2 Antibody.)

Western Blot (WB)

(Western blot analysis of GNRHR2 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of GNRHR2 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9604309 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9604309 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-GNRHR2 antibody
Function: Putative receptor for gonadotropin releasing hormone II (GnRH II) which is most probably non-functional.
Post-translational Modifications: Phosphorylated on the C-terminal cytoplasmic tail.
Similarity: Belongs to the G-protein coupled receptor 1 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 42 kDa
Predicted: 33 kDa
NCBI Official Full Name
Putative gonadotropin-releasing hormone II receptor
NCBI Official Synonym Full Names
gonadotropin releasing hormone receptor 2 (pseudogene)
NCBI Official Symbol
GNRHR2
NCBI Official Synonym Symbols
GnRH-II-R
UniProt Protein Name
Putative gonadotropin-releasing hormone II receptor
UniProt Gene Name
GNRHR2
UniProt Synonym Gene Names
GnRH II receptor; GnRH-II-R

NCBI Description

In non-hominoid primates and non-mammalian vertebrates, the gonadotropin releasing hormone 2 receptor gene (GnRHR2) encodes a seven-transmembrane G-protein coupled receptor. However, in human, the corresponding reading frame contains a premature stop codon, which has been suggested to encode a selenocysteine residue, but there is no solid evidence for selenocysteine incorporation (PMID: 12538601). It appears that the human GnRHR2 transcription occurs but the gene does not likely produce a functional multi-transmembrane protein. A non-transcribed pseudogene of GnRHR2 is located on chromosome 14. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

Putative receptor for gonadotropin releasing hormone II (GnRH II) which is most probably non-functional.

Research Articles on GNRHR2

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Product Notes

The GNRHR2 gnrhr2 (Catalog #AAA9604309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GNRHR2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GNRHR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the GNRHR2 gnrhr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GNRHR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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