Western Blot (WB)
((1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PERP Polyclonal Antibody | anti-PERP antibody
Goat anti-PERP Antibody
Gene Names
PERP; THW; KCP1; PIGPC1; KRTCAP1; dJ496H19.1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PERP; Polyclonal Antibody; Goat anti-PERP Antibody; TP53 apoptosis effector; HGNC: 17637; KCP1; KRTCAP1; PIGPC1; THW; dJ496H19.1; 1110017A08Rik; RP3-496H19.1; keratinocytes associated protein 1; p53-induced protein PIGPC1; PERP antibody; TP53 apoptosis effector antibody; HGNC: 17637 antibody; KCP1 antibody; KRTCAP1 antibody; PIGPC1 antibody; THW antibody; dJ496H19.1 antibody; 1110017A08Rik antibody; RP3-496H19.1 antibody; keratinocytes associated protein 1 antibody; p53-induced protein PIGPC1 antibody; anti-PERP antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
CLPNYEDDLLGNAK
Sequence Length
193
Applicable Applications for anti-PERP antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 20kDa band observed in human liver lysates (calculated MW of 21.4kDa according to NP_071404.2). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 20kDa band observed in human liver lysates (calculated MW of 21.4kDa according to NP_071404.2). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence CLPNYEDDLLGNAK, from the C Terminus of the protein sequence according to NP_071404.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,386 Da
NCBI Official Full Name
p53 apoptosis effector related to PMP-22
NCBI Official Synonym Full Names
PERP, TP53 apoptosis effector
NCBI Official Symbol
PERP
NCBI Official Synonym Symbols
THW; KCP1; PIGPC1; KRTCAP1; dJ496H19.1
NCBI Protein Information
p53 apoptosis effector related to PMP-22
UniProt Protein Name
p53 apoptosis effector related to PMP-22
Protein Family
UniProt Gene Name
PERP
UniProt Synonym Gene Names
KCP1; KRTCAP1; PIGPC1; THW; KCP-1
UniProt Entry Name
PERP_HUMAN
Uniprot Description
PERP: Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway. Belongs to the TMEM47 family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell adhesion; Mitochondrial
Chromosomal Location of Human Ortholog: 6q24
Cellular Component: cell junction; desmosome; Golgi apparatus; integral to plasma membrane; mitochondrion; plasma membrane
Biological Process: heterotypic cell-cell adhesion; Notch signaling pathway; positive regulation of proteolysis; regulation of apoptosis
Research Articles on PERP
Similar Products
Product Notes
The PERP perp (Catalog #AAA420946) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PERP Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's PERP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 20kDa band observed in human liver lysates (calculated MW of 21.4kDa according to NP_071404.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the PERP perp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CLPNYEDDLL GNAK. It is sometimes possible for the material contained within the vial of "PERP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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