Immunohistochemistry (IHC)
(MBS9604310 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse GPR110 Polyclonal Antibody | anti-GPR110 antibody
GPR110 Antibody
Gene Names
ADGRF1; PGR19; GPR110; KPG_012; hGPCR36
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GPR110; Polyclonal Antibody; GPR110 Antibody; G protein coupled receptor 110; G protein coupled receptor KPG_012; G protein coupled receptor PGR19; G-protein coupled receptor KPG_012; G-protein coupled receptor PGR19; GP110_HUMAN; HGPCR36; KPG_012; PGR19; Probable G protein coupled receptor 110; Probable G-protein coupled receptor 110; anti-GPR110 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
GPR110 antibody detects endogenous levels of total GPR110
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
218
Applicable Applications for anti-GPR110 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Tissue Specificity
Mainly expressed in the kidney. Up-regulated in lung adenocarcinomas and prostate cancers.
Predicted Cross Reactivity
Rabbit
Similarity
Rabbit (85%)
Conjugation
Unconjugated
Immunohistochemistry (IHC)
(MBS9604310 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604310 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of GPR110 expression in Jurkat cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of GPR110 expression in Jurkat cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescence (IF)
(MBS9604310 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9604310 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-GPR110 antibody
Description: Orphan receptor.
Post-translational Modifications: Glycosylated.
Similarity: Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Post-translational Modifications: Glycosylated.
Similarity: Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 101 kDa
Predicted: 102 kDa
Predicted: 102 kDa
NCBI Official Full Name
adhesion G-protein coupled receptor F1 isoform 2
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor F1
NCBI Official Symbol
ADGRF1
NCBI Official Synonym Symbols
PGR19; GPR110; KPG_012; hGPCR36
NCBI Protein Information
adhesion G-protein coupled receptor F1
UniProt Protein Name
Adhesion G-protein coupled receptor F1
UniProt Gene Name
ADGRF1
Uniprot Description
Orphan receptor.
Research Articles on GPR110
Similar Products
Product Notes
The GPR110 adgrf1 (Catalog #AAA9604310) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR110 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GPR110 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the GPR110 adgrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR110, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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