Rabbit anti-Mouse Gnl3 Polyclonal Antibody | anti-Gnl3 antibody

Gnl3, CT (Gnl3, Ns, Guanine nucleotide-binding protein-like 3, Nucleolar GTP-binding protein 3, Nucleostemin) (PE)

Cat. Num. AAA6303083

• Gene Names: Gnl3; Ns
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Gnl3; Polyclonal Antibody; CT (Gnl3; Ns; Guanine nucleotide-binding protein-like 3; Nucleolar GTP-binding protein 3; Nucleostemin) (PE); Nucleostemin; anti-Gnl3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Gnl3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Gnl3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-471 amino acids from the C-terminal region of mouse Gnl3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Gnl3 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Mouse Gnl3 antibody detected the Mouse Gnl3 protein (arrow).)

Related Product Information for anti-Gnl3 antibody

Gnl3 may be required to maintain the proliferative capacity of stem cells (By similarity).

Product Categories/Family for anti-Gnl3 antibody

Antibodies; Binding Proteins

References

Hirai, H., et al. Biochem. Biophys. Res. Commun. 391(1):299-304(2010). Tamase, A., et al. Proc. Natl. Acad. Sci. U.S.A. 106(40):17163-17168(2009). Nomura, J., et al. Stem Cells 27(5):1066-1076(2009). Ohmura, M., et al. Stem Cells 26(12):3237-3246(2008). Siddiqi, S., et al. Circ. Res. 103(1):89-97(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 30524920
• NCBI GeneID: 30877
• NCBI Accession #: NP_705775.2
• NCBI GenBank Nucleotide #: NM_153547.6
• UniProt Accession #: Q8CI11; Q811R9; Q811S8; Q8BK21
• Molecular Weight: 60,786 Da
• NCBI Official Full Name: guanine nucleotide-binding protein-like 3
• NCBI Official Synonym Full Names: guanine nucleotide binding protein-like 3 (nucleolar)
• NCBI Official Symbol: Gnl3
• NCBI Official Synonym Symbols: Ns
• NCBI Protein Information: guanine nucleotide-binding protein-like 3; nucleostemin; nucleolar GTP-binding protein 3
• UniProt Protein Name: Guanine nucleotide-binding protein-like 3
• Protein Family: Guanine nucleotide-binding protein
• UniProt Gene Name: Gnl3
• UniProt Synonym Gene Names: Ns
• UniProt Entry Name: GNL3_MOUSE

Uniprot Description

Function: May be required to maintain the proliferative capacity of stem cells

By similarity. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation. Ref.5

Subunit structure: Interacts with MDM2; this interaction stabilizes MDM2. Interaction with MDM2 occurs in the nucleoplasm and is triggered by a nucleolar release mechanism, such as mitosis-induced nucleolar disassembly. Indirectly interacts with TP53, via MDM2-binding

By similarity. Ref.5

Subcellular location: Nucleus

By similarity. Nucleus › nucleolus

By similarity. Note: Shuttles between the nucleus and nucleolus

By similarity. Ref.5

Tissue specificity: Expressed in the adult bone marrow population that is enriched in hematopoietic stem cells. Ref.1

Developmental stage: Expressed at E8.5 and E10.5 in the cerebral cortex; expression declines rapidly from this point. Ref.1

Domain: The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis

By similarity.In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similarities: Belongs to the MMR1/HSR1 GTP-binding protein family.