Rabbit anti-Mouse Gnl3 Polyclonal Antibody | anti-Gnl3 antibody

Gnl3, CT (Gnl3, Ns, Guanine nucleotide-binding protein-like 3, Nucleolar GTP-binding protein 3, Nucleostemin) (Azide free) (HRP)

Cat. Num. AAA6303077

• Gene Names: Gnl3; Ns
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Gnl3; Polyclonal Antibody; CT (Gnl3; Ns; Guanine nucleotide-binding protein-like 3; Nucleolar GTP-binding protein 3; Nucleostemin) (Azide free) (HRP); Nucleostemin; anti-Gnl3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Gnl3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Gnl3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-471 amino acids from the C-terminal region of mouse Gnl3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Gnl3 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Mouse Gnl3 antibody detected the Mouse Gnl3 protein (arrow).)

Related Product Information for anti-Gnl3 antibody

Gnl3 may be required to maintain the proliferative capacity of stem cells (By similarity).

Product Categories/Family for anti-Gnl3 antibody

Antibodies; Binding Proteins

References

Hirai, H., et al. Biochem. Biophys. Res. Commun. 391(1):299-304(2010). Tamase, A., et al. Proc. Natl. Acad. Sci. U.S.A. 106(40):17163-17168(2009). Nomura, J., et al. Stem Cells 27(5):1066-1076(2009). Ohmura, M., et al. Stem Cells 26(12):3237-3246(2008). Siddiqi, S., et al. Circ. Res. 103(1):89-97(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 30524920
• NCBI GeneID: 30877
• NCBI Accession #: NP_705775.2
• NCBI GenBank Nucleotide #: NM_153547.6
• UniProt Accession #: Q8CI11; Q811R9; Q811S8; Q8BK21
• Molecular Weight: 60,786 Da
• NCBI Official Full Name: guanine nucleotide-binding protein-like 3
• NCBI Official Synonym Full Names: guanine nucleotide binding protein-like 3 (nucleolar)
• NCBI Official Symbol: Gnl3
• NCBI Official Synonym Symbols: Ns
• NCBI Protein Information: guanine nucleotide-binding protein-like 3; nucleostemin; nucleolar GTP-binding protein 3
• UniProt Protein Name: Guanine nucleotide-binding protein-like 3
• Protein Family: Guanine nucleotide-binding protein
• UniProt Gene Name: Gnl3
• UniProt Synonym Gene Names: Ns
• UniProt Entry Name: GNL3_MOUSE

Uniprot Description

Function: May be required to maintain the proliferative capacity of stem cells

By similarity. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation. Ref.5

Subunit structure: Interacts with MDM2; this interaction stabilizes MDM2. Interaction with MDM2 occurs in the nucleoplasm and is triggered by a nucleolar release mechanism, such as mitosis-induced nucleolar disassembly. Indirectly interacts with TP53, via MDM2-binding

By similarity. Ref.5

Subcellular location: Nucleus

By similarity. Nucleus › nucleolus

By similarity. Note: Shuttles between the nucleus and nucleolus

By similarity. Ref.5

Tissue specificity: Expressed in the adult bone marrow population that is enriched in hematopoietic stem cells. Ref.1

Developmental stage: Expressed at E8.5 and E10.5 in the cerebral cortex; expression declines rapidly from this point. Ref.1

Domain: The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis

By similarity.In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similarities: Belongs to the MMR1/HSR1 GTP-binding protein family.