Goat GNB2L1 / RACK1 Polyclonal Antibody | anti-GNB2L1 antibody

Goat Polyclonal to Human GNB2L1 / RACK1

Cat. Num. AAA244754

• Gene Names: GNB2L1; H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
• Reactivity: Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rat; Predicted Reactivity: Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: GNB2L1 / RACK1; Polyclonal Antibody; Goat Polyclonal to Human GNB2L1 / RACK1; Anti-GNB2L1 / RACK1 Antibody (Internal) IHC-plus; GNB2L1; H12.3; Gnb2-rs1; Proliferation-inducing gene 21; RACK1; HLC-7; Lung cancer oncogene 7; PIG21; Human GNB2L1; anti-GNB2L1 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rat; Predicted Reactivity: Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human GNB2L1 / RACK1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 317

• Applicable Applications for anti-GNB2L1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 33kD band observed in Human Lymph Nodes and Mouse and Rat Spleen lysates (calculated MW of 35.1kD according to NP_006089.1). Recommended concentration: 0.1-0.3 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide NTLGVCKYTVQDESH from an internal region of human GNB2L1 / RACK1 (NP_006089.1).
• Immunogen Type: Synthetic peptide
• Immunogen: GNB2L1 / RACK1 antibody was raised against synthetic peptide NTLGVCKYTVQDESH from an internal region of human GNB2L1 / RACK1 (NP_006089.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus (100%); Rabbit, Salmon, Smelt, Pike (93%); Catfish, Sablefish, Stickleback, Pufferfish, Zebrafish (87%); Sea anemone (80%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-GNB2L1 / RACK1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)

Western Blot (WB)

(GNB2L1 antibody (0.1 ug/ml) staining of Human Lymph Node lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 5174447
• NCBI GeneID: 10399
• NCBI Accession #: NP_006089.1
• NCBI GenBank Nucleotide #: NM_006098.4
• UniProt Accession #: P63244; P25388; P99049; Q53HU2; Q5J8M6; Q5VLR4; Q6FH47; B3KTJ0; D3DWS0
• Molecular Weight: 35,077 Da
• NCBI Official Full Name: guanine nucleotide-binding protein subunit beta-2-like 1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• NCBI Official Symbol: GNB2L1
• NCBI Official Synonym Symbols: H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
• NCBI Protein Information: guanine nucleotide-binding protein subunit beta-2-like 1; lung cancer oncogene 7; proliferation-inducing gene 21; receptor for activated C kinase 1; human lung cancer oncogene 7 protein; receptor of activated protein kinase C 1; cell proliferation-inducing gene 21 protein; guanine nucleotide binding protein beta polypeptide 2-like 1; guanine nucleotide-binding protein subunit beta-like protein 12.3; protein homologous to chicken B complex protein, guanine nucleotide binding
• UniProt Protein Name: Guanine nucleotide-binding protein subunit beta-2-like 1
• UniProt Gene Name: GNB2L1
• UniProt Synonym Gene Names: HLC-7; RACK1
• UniProt Entry Name: GBLP_HUMAN

Uniprot Description

RACK1: a G-beta like protein containing 7 WD domains. A presumptive scaffold protein, linking protein kinase C to its substrates. Binds inositol 1,4,5-trisphosphate receptors and regulates Ca2+ release by enhancing inositol 1,4,5-trisphosphate receptor binding affinity for inositol 1,4,5-trisphosphate. An insulin-like growth factor 1 receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death.

Protein type: G protein, heterotrimeric beta; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Adaptor/scaffold; G protein, heterotrimeric; G protein

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: small ribosomal subunit; cytoskeleton; mitochondrion; cell soma; perinuclear region of cytoplasm; dendrite; cytoplasm; perikaryon; midbody; nucleus; cytosol; phagocytic cup

Molecular Function: protein tyrosine kinase inhibitor activity; protein binding; protein homodimerization activity; enzyme binding; protein kinase C binding; protein complex scaffold; SH2 domain binding; caspase activator activity; receptor tyrosine kinase binding; protein phosphatase binding; ion channel inhibitor activity; receptor binding

Biological Process: caspase activation; negative regulation of phagocytosis; negative regulation of protein kinase B signaling cascade; negative regulation of Wnt receptor signaling pathway; viral reproduction; positive regulation of protein homooligomerization; regulation of cell cycle; positive regulation of apoptosis; rhythmic process; gastrulation; cell cycle; osteoblast differentiation; regulation of protein localization; positive regulation of mitochondrial depolarization; negative regulation of translation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cAMP catabolic process; positive regulation of cyclic-nucleotide phosphodiesterase activity; negative regulation of cell growth; positive regulation of protein amino acid phosphorylation; regulation of cell division; positive regulation of cell migration; positive regulation of GTPase activity