Rabbit anti-Human GNB2L1 Polyclonal Antibody | anti-GNB2L1 antibody

GNB2L1, NT (Guanine Nucleotide Binding Protein Subunit beta-2-like 1, Guanine Nucleotide-binding Protein Subunit beta-like Protein 12.3, Cell Proliferation-inducing Gene 21 Protein, Gnb2-rs1, H12.3, Human Lung Cancer Oncogene 7 Protein, HLC7, HLC-7, PIG21

Cat. Num. AAA6478673

• Gene Names: RACK1; H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: GNB2L1; Polyclonal Antibody; NT (Guanine Nucleotide Binding Protein Subunit beta-2-like 1; Guanine Nucleotide-binding Protein Subunit beta-like Protein 12.3; Cell Proliferation-inducing Gene 21 Protein; Gnb2-rs1; H12.3; Human Lung Cancer Oncogene 7 Protein; HLC7; HLC-7; PIG21; anti-GNB2L1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GNB2L1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GNB2L1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human GNB2L1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-GNB2L1 Pab is used in Western blot to detect GNB2L1 in Ramos cell lysate.)

Western Blot (WB)

(Western blot analysis of GNB2L1 (arrow) using GNB2L1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GNB2L1 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-GNB2L1 antibody

Antibodies; Protein Kinases

References

1. Ceci, M., et al., Nature 426(6966):579-584 (2003). 2. Usacheva, A., et al., J. Immunol. 171(6):2989-2994 (2003). 3. Kiely, P.A., et al., J. Biol. Chem. 277(25):22581-22589 (2002). 4. Tcherkasowa, A.E., et al., J. Immunol. 169(9):5161-5170 (2002). 5. Liedtke, C.M., et al., J. Biol. Chem. 277(25):22925-22933 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 5174447
• NCBI GeneID: 10399
• NCBI Accession #: NP_006089
• NCBI GenBank Nucleotide #: NM_006098.4
• UniProt Accession #: P63244; P25388; P99049; Q53HU2; Q5J8M6; Q5VLR4; Q6FH47; B3KTJ0; D3DWS0
• Molecular Weight: 37.2 kDa (337aa) confirmed by MALDI-TOF
• NCBI Official Full Name: receptor of activated protein C kinase 1
• NCBI Official Synonym Full Names: receptor for activated C kinase 1
• NCBI Official Symbol: RACK1
• NCBI Official Synonym Symbols: H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
• NCBI Protein Information: receptor of activated protein C kinase 1
• UniProt Protein Name: Guanine nucleotide-binding protein subunit beta-2-like 1
• UniProt Gene Name: GNB2L1
• UniProt Synonym Gene Names: HLC-7; RACK1
• UniProt Entry Name: GBLP_HUMAN