Rabbit anti-Mouse Brsk2 Polyclonal Antibody | anti-Brsk2 antibody

Brsk2, ID (Brsk2, Kiaa4256, Sada, Serine/threonine-protein kinase BRSK2, Brain-specific serine/threonine-protein kinase 2, Serine/threonine-protein kinase SAD-A) (Biotin)

Cat. Num. AAA6279002

• Gene Names: Brsk2; SADA; SAD-A; 4833424K13Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Brsk2; Polyclonal Antibody; ID (Brsk2; Kiaa4256; Sada; Serine/threonine-protein kinase BRSK2; Brain-specific serine/threonine-protein kinase 2; Serine/threonine-protein kinase SAD-A) (Biotin); Serine/threonine-protein kinase SAD-A; anti-Brsk2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 719

• Applicable Applications for anti-Brsk2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Brsk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 328-356 amino acids from the Central region of Mouse Brsk2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Brsk2 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Brsk2 antibody detected the Brsk2 protein (arrow).)

Related Product Information for anti-Brsk2 antibody

Brsk2 is required for the polarization of forebrain neurons which endows axons and dendrites with distinct properties, possibly by locally regulating phosphorylation of microtubule-associated proteins.

Product Categories/Family for anti-Brsk2 antibody

Antibodies; Protein Kinases

References

Muller, M., et al. J. Cell. Sci. 123 (PT 2), 286-294 (2010): Choi, Y.J., et al. Genes Dev. 22(18):2485-2495(2008). Hezel, A.F., et al. Mol. Cell. Biol. 28(7):2414-2425(2008). Barnes, A.P., et al. Cell 129(3):549-563(2007). Munton, R.P., et al. Mol. Cell Proteomics 6(2):283-293(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 58036485
• NCBI GeneID: 75770
• NCBI Accession #: NP_001009930.1; NP_083702.1
• NCBI GenBank Nucleotide #: NP_001009930.1
• UniProt Accession #: Q69Z98
• NCBI Official Full Name: serine/threonine-protein kinase BRSK2 isoform gamma
• NCBI Official Synonym Full Names: BR serine/threonine kinase 2
• NCBI Official Symbol: Brsk2
• NCBI Official Synonym Symbols: SADA; SAD-A; 4833424K13Rik
• NCBI Protein Information: serine/threonine-protein kinase BRSK2
• UniProt Protein Name: Serine/threonine-protein kinase BRSK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Brsk2
• UniProt Synonym Gene Names: Kiaa4256; Sada; BR serine/threonine-protein kinase 2

Uniprot Description

BRSK2: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. BRSK1 and BRSK2 are required for nueronal polarization. Expressed in the brain and at low levels in the testis. Four alternatively spliced isoforms have been described.

Protein type: BRSK subfamily; CAMK group; CAMKL family; EC 2.7.11.1; EC 2.7.11.26; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 7|7 F5

Cellular Component: centrosome; cytoplasm; cytoskeleton; endoplasmic reticulum; intracellular; nucleus

Molecular Function: ATP binding; ATPase binding; kinase activity; magnesium ion binding; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau-protein kinase activity; transferase activity

Biological Process: actin cytoskeleton reorganization; apoptosis; axonogenesis; cell cycle; cell division; establishment of cell polarity; exocytosis; G2/M transition of mitotic cell cycle; nervous system development; neuron differentiation; neuron projection morphogenesis; peptidyl-serine phosphorylation; phosphorylation; protein amino acid phosphorylation