Rabbit GNB1 Polyclonal Antibody | anti-GNB1 antibody

Anti-GNB1 Antibody

Cat. Num. AAA178823

• Gene Names: GNB1; MRD42
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: GNB1; Polyclonal Antibody; Anti-GNB1 Antibody; G protein beta 1 subunit; GBB1; gnb1; P62873; Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; G protein subunit beta 1; anti-GNB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 240

• Applicable Applications for anti-GNB1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot: 0.1-0.5ug/ml; Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml
• Notes: Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GNB1 (2-42aa SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR), identical to the related mouse and rat sequences.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of GNB1 expression in rat liver extract (lane 1), mouse cardiac muscle extract (lane 2) and HELA whole cell lysates (lane 3). GNB1 at 37KD was detected using rabbit anti- GNB1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Immunohistochemistry (IHC)

(GNB1 was detected in paraffin-embedded sections of mouse kidney tissues using rabbit anti- GNB1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(GNB1 was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- GNB1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(GNB1 was detected in paraffin-embedded sections of human liver cancer tissues using rabbit anti- GNB1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Related Product Information for anti-GNB1 antibody

Rabbit IgG polyclonal antibody for Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1(GNB1) detection.
Background: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 is a protein that in humans is encoded by the GNB1 gene. Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals.

References

1. Codina J, Stengel D, Woo SL, Birnbaumer L (1986). "Beta-subunits of the human liver Gs/Gi signal-transducing proteins and those of bovine retinal rod cell transducin are identical". FEBS Lett. 207 (2): 187-92.
2. Yan K, Kalyanaraman V, Gautam N (March 1996). "Differential ability to form the G protein betagamma complex among members of the beta and gamma subunit families". J. Biol. Chem. 271 (12): 7141-6.

NCBI and Uniprot Product Information

• NCBI GI #: 543173167
• NCBI GeneID: 2782
• NCBI Accession #: NP_001269467.1
• NCBI GenBank Nucleotide #: NM_001282538.1
• UniProt Accession #: P62873; P04697; P04901; Q1RMY8; B1AJZ7
• Molecular Weight: 36,298 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 isoform 2
• NCBI Official Synonym Full Names: G protein subunit beta 1
• NCBI Official Symbol: GNB1
• NCBI Official Synonym Symbols: MRD42
• NCBI Protein Information: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Protein Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Gene Name: GNB1

NCBI Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

G-beta 1: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the WD repeat G protein beta family.

Protein type: G protein; G protein, heterotrimeric; G protein, heterotrimeric beta

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cytosol; heterotrimeric G-protein complex; intracellular; lysosomal membrane; membrane; plasma membrane

Molecular Function: GTPase activity; GTPase binding; protein binding; protein complex binding

Biological Process: acetylcholine receptor signaling, muscarinic pathway; dopamine receptor, adenylate cyclase activating pathway; platelet activation; protein folding; Ras protein signal transduction; rhodopsin mediated signaling; signal transduction; Wnt receptor signaling pathway, calcium modulating pathway

Disease: Leukemia, Acute Lymphoblastic; Mental Retardation, Autosomal Dominant 42