Rabbit anti-Human GNAT3 Polyclonal Antibody | anti-GNAT3 antibody

GNAT3, ID (GNAT3, Guanine nucleotide-binding protein G(t) subunit alpha-3, Gustducin alpha-3 chain) (AP)

Cat. Num. AAA6302974

• Gene Names: GNAT3; GDCA
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: GNAT3; Polyclonal Antibody; ID (GNAT3; Guanine nucleotide-binding protein G(t) subunit alpha-3; Gustducin alpha-3 chain) (AP); Gustducin alpha-3 chain; anti-GNAT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GNAT3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-GNAT3 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 78-105 in the central region of human GNAT3.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A549 cell line lysates (35ug/lane) using MBS6050334. This demonstrates that MBS6050334 detected the GNAT3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma using MBS6050334 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6050334 for immunohistochemistry.)

Testing Data

Product Categories/Family for anti-GNAT3 antibody

Antibodies; Binding Proteins

References

1. Fushan, A.A., et al. Chem. Senses 35(7):579-592 (2010). 2. Luttrell, L.M. Mol. Biotechnol. 39(3):239-264 (2008). 3. Margolskee, R.F., et al. Proc. Natl. Acad. Sci. U.S.A. 104(38):15075-15080 (2007). 4. Jang, H.J., et al. Proc. Natl. Acad. Sci. U.S.A. 104(38):15069-15074 (2007). 5. Fehr, J., et al. J. Comp. Physiol. A Neuroethol. Sens. Neural. Behav. Physiol. 193(1):21-34 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 156139155
• NCBI GeneID: 346562
• NCBI Accession #: NP_001095856.1
• NCBI GenBank Nucleotide #: NM_001102386.1
• UniProt Accession #: A8MTJ3; A4D1B2; A4D1B3; B9EJG5
• Molecular Weight: 40,357 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(t) subunit alpha-3
• NCBI Official Synonym Full Names: guanine nucleotide binding protein, alpha transducing 3
• NCBI Official Symbol: GNAT3
• NCBI Official Synonym Symbols: GDCA
• NCBI Protein Information: guanine nucleotide-binding protein G(t) subunit alpha-3; guanine nucleotide-binding protein G(t) subunit alpha-3; gustducin alpha-3 chain
• UniProt Protein Name: Guanine nucleotide-binding protein G(t) subunit alpha-3
• UniProt Gene Name: GNAT3
• UniProt Entry Name: GNAT3_HUMAN

Uniprot Description

G-alpha t3: Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide- 1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity. Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Protein type: G protein, heterotrimeric; G protein; G protein, heterotrimeric alpha G((i/o/t/z))

Chromosomal Location of Human Ortholog: 7q21.11

Cellular Component: protein complex; photoreceptor inner segment; photoreceptor outer segment; apical plasma membrane; plasma membrane; acrosome; heterotrimeric G-protein complex; axoneme

Molecular Function: GTPase activity; G-protein-coupled receptor binding; GTP binding; metal ion binding; G-protein beta/gamma-subunit binding; G-protein coupled photoreceptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; response to nicotine; platelet activation; synaptic transmission; sensory perception of umami taste; sensory perception of sweet taste; metabolic process; detection of chemical stimulus involved in sensory perception of bitter taste; G-protein signaling, adenylate cyclase inhibiting pathway; blood coagulation; detection of visible light