Rabbit GNAT1 Polyclonal Antibody | anti-GNAT1 antibody

GNAT1 Antibody (C-term)

Cat. Num. AAA9202117

• Gene Names: GNAT1; GBT1; GNATR; CSNB1G; CSNBAD3
• Reactivity: Human (Predicted Reactivity: Mouse, Xenopus)
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GNAT1; Polyclonal Antibody; GNAT1 Antibody (C-term); Guanine nucleotide-binding protein G(t) subunit alpha-1; Transducin alpha-1 chain; GNATR; anti-GNAT1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse, Xenopus)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This GNAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-318 amino acids from the C-terminal region of human GNAT1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 290-318
• Sequence Length: 350

• Applicable Applications for anti-GNAT1 antibody: WB, IHC-P, FC, E
• Application Notes: WB~~1:1000; IHC-P~~1:50~100; FC~~1:10~50
• Function: Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (PubMed:22190596). Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).
• Cellular Location: Cell projection, cilium, photoreceptor outer segment {ECO:0000250|UniProtKB:P04695}. Membrane {ECO:0000250|UniProtKB:P04695}; Peripheral membrane protein {ECO:0000250|UniProtKB:P04695}
• Tissue Location: Rod photoreceptor cells (PubMed:1614872). Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium (PubMed:22190596)
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of GNAT1 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). GNAT1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(GNAT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GNAT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GNAT1 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GNAT1 antibody

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells.

Product Categories/Family for anti-GNAT1 antibody

Neuroscience; Signal Transduction

References

Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008)
Szabo, V., et al. Hum. Mutat. 28(7):741-742(2007)
Yi, H.M., et al. Ai Zheng 26(1):9-14(2007)
Oldham, W.M., et al. Nat. Struct. Mol. Biol. 13(9):772-777(2006)
Muzny, D.M., et al. Nature 440(7088):1194-1198(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 22027520
• NCBI GeneID: 2779
• NCBI Accession #: NP_000163.2; NP_653082.1
• NCBI GenBank Nucleotide #: NM_000172.3
• UniProt Accession #: P11488; Q4VBN2
• NCBI Official Full Name: guanine nucleotide-binding protein G(t) subunit alpha-1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1
• NCBI Official Symbol: GNAT1
• NCBI Official Synonym Symbols: GBT1; GNATR; CSNB1G; CSNBAD3
• NCBI Protein Information: guanine nucleotide-binding protein G(t) subunit alpha-1
• UniProt Protein Name: Guanine nucleotide-binding protein G(t) subunit alpha-1
• UniProt Gene Name: GNAT1
• UniProt Synonym Gene Names: GNATR
• UniProt Entry Name: GNAT1_HUMAN

NCBI Description

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009]

Uniprot Description

G-alpha t1: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3); also known as congenital stationary night blindness Nougaret type. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Protein type: G protein, heterotrimeric; G protein; G protein, heterotrimeric alpha G((i/o/t/z))

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: photoreceptor outer segment; photoreceptor inner segment; membrane; cell soma; apical plasma membrane; plasma membrane; heterotrimeric G-protein complex; cytosol; photoreceptor connecting cilium

Molecular Function: GTPase activity; signal transducer activity; GDP binding; acyl binding; GTP binding; G-protein-coupled receptor binding; metal ion binding; G-protein beta/gamma-subunit binding; protein kinase binding

Biological Process: response to light stimulus; phototransduction, visible light; sensory perception of umami taste; metabolic process; response to light intensity; detection of chemical stimulus involved in sensory perception of bitter taste; signal transduction; rhodopsin mediated signaling; G-protein signaling, coupled to cAMP nucleotide second messenger; cell proliferation; eye photoreceptor cell development; regulation of rhodopsin mediated signaling; visual perception; retina development in camera-type eye; detection of light stimulus involved in visual perception; positive regulation of cyclic-nucleotide phosphodiesterase activity; negative regulation of cyclic-nucleotide phosphodiesterase activity

Disease: Night Blindness, Congenital Stationary, Type 1g; Night Blindness, Congenital Stationary, Autosomal Dominant 3