Rabbit anti-Human GNAT1 Polyclonal Antibody | anti-GNAT1 antibody

GNAT1, CT (GNAT1, GNATR, Guanine nucleotide-binding protein G(t) subunit alpha-1, Transducin alpha-1 chain) (Azide free) (HRP)

Cat. Num. AAA6302956

• Gene Names: GNAT1; GBT1; GNATR; CSNBAD3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GNAT1; Polyclonal Antibody; CT (GNAT1; GNATR; Guanine nucleotide-binding protein G(t) subunit alpha-1; Transducin alpha-1 chain) (Azide free) (HRP); Transducin alpha-1 chain; anti-GNAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GNAT1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GNAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297~326 amino acids from the C-terminal region of human GNAT1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GNAT1 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). GNAT1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(GNAT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded moouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GNAT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GNAT1 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GNAT1 antibody

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells.

Product Categories/Family for anti-GNAT1 antibody

Antibodies; G Proteins

References

Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008). Szabo, V., et al. Hum. Mutat. 28(7):741-742(2007). Yi, H.M., et al. Ai Zheng 26(1):9-14(2007). Oldham, W.M., et al. Nat. Struct. Mol. Biol. 13(9):772-777(2006). Muzny, D.M., et al. Nature 440(7088):1194-1198(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 22027520
• NCBI GeneID: 2779
• NCBI Accession #: NP_000163.2
• NCBI GenBank Nucleotide #: NM_000172.3
• UniProt Accession #: P11488; Q4VBN2
• Molecular Weight: 40,041 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(t) subunit alpha-1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1
• NCBI Official Symbol: GNAT1
• NCBI Official Synonym Symbols: GBT1; GNATR; CSNBAD3
• NCBI Protein Information: guanine nucleotide-binding protein G(t) subunit alpha-1; guanine nucleotide-binding protein G(t) subunit alpha-1; transducin alpha-1 chain; transducin, rod-specific; rod-type transducin alpha subunit; guanine nucleotide-binding protein G(T), alpha-1 subun
• UniProt Protein Name: Guanine nucleotide-binding protein G(t) subunit alpha-1
• UniProt Gene Name: GNAT1
• UniProt Synonym Gene Names: GNATR
• UniProt Entry Name: GNAT1_HUMAN

NCBI Description

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009]

Uniprot Description

G-alpha t1: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3); also known as congenital stationary night blindness Nougaret type. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Protein type: G protein, heterotrimeric alpha G((i/o/t/z)); G protein; G protein, heterotrimeric

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: photoreceptor outer segment; photoreceptor inner segment; membrane; cell soma; apical plasma membrane; plasma membrane; heterotrimeric G-protein complex; cytosol; photoreceptor connecting cilium

Molecular Function: GTPase activity; signal transducer activity; GDP binding; acyl binding; G-protein-coupled receptor binding; GTP binding; metal ion binding; G-protein beta/gamma-subunit binding; protein kinase binding

Biological Process: phototransduction, visible light; response to light stimulus; sensory perception of umami taste; metabolic process; response to light intensity; detection of chemical stimulus involved in sensory perception of bitter taste; signal transduction; rhodopsin mediated signaling; G-protein signaling, coupled to cAMP nucleotide second messenger; cell proliferation; eye photoreceptor cell development; regulation of rhodopsin mediated signaling; visual perception; retina development in camera-type eye; detection of light stimulus involved in visual perception; positive regulation of cyclic-nucleotide phosphodiesterase activity; negative regulation of cyclic-nucleotide phosphodiesterase activity

Disease: Night Blindness, Congenital Stationary, Type 1g; Night Blindness, Congenital Stationary, Autosomal Dominant 3