Rabbit anti-Human ADRB2 Polyclonal Antibody | anti-ADRB2 antibody

ADRB2 (S364) (ADRB2R, ADRBR, B2AR, BAR, Beta-2 Adrenergic Receptor, Beta-2 Adrenoceptor, Beta-2 Adrenoreceptor, BETA2AR, Adrenergic, beta-2-, Receptor, Surface) (AP)

Cat. Num. AAA6461797

• Gene Names: ADRB2; BAR; B2AR; ADRBR; ADRB2R; BETA2AR
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ADRB2; Polyclonal Antibody; ADRB2 (S364) (ADRB2R; ADRBR; B2AR; BAR; Beta-2 Adrenergic Receptor; Beta-2 Adrenoceptor; Beta-2 Adrenoreceptor; BETA2AR; Adrenergic; beta-2-; Receptor; Surface) (AP); anti-ADRB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADRB2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ADRB2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 345-373 of human ADRB2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(All lanes: MBS626831 (1:1000), lane 1: Hela whole cell lysate, lane 2: HepG2 whole cell lysate, lane 3: HUVEC whole cell lysate, lane 4: Human liver lysate, lane 5: NCI-H460 whole cell lysate, lane 6: PC-3 whole cell lysate, lane 7: A431 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 46kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

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Testing Data

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Related Product Information for anti-ADRB2 antibody

ADRB2, beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This protein is intronless.

Product Categories/Family for anti-ADRB2 antibody

Antibodies; Receptors

References

1. Kobilka B.K., Dixon R.A.F.Proc. Natl. Acad. Sci. USA 84:46-50(1987). 2. Emorine L.J., Marullo S.Proc. Natl. Acad. Sci. USA 84:6995-6999(1987) 3. Kobilka B.K., Frielle T.J. Biol. Chem. 262:7321-7327(1987).

NCBI and Uniprot Product Information

• NCBI GI #: 4501969
• NCBI GeneID: 154
• NCBI Accession #: NP_000015
• NCBI GenBank Nucleotide #: NM_000024
• UniProt Accession #: P07550; O14823; O14824; O14825; O14826; Q4JG18; Q53GA6; Q6GMT4; Q6P4D8; Q8NEQ9; B0LPE4; B2R7X2
• Molecular Weight: 46,459 Da
• NCBI Official Full Name: beta-2 adrenergic receptor
• NCBI Official Synonym Full Names: adrenoceptor beta 2, surface
• NCBI Official Symbol: ADRB2
• NCBI Official Synonym Symbols: BAR; B2AR; ADRBR; ADRB2R; BETA2AR
• NCBI Protein Information: beta-2 adrenergic receptor; adrenergic, beta-2-, receptor, surface; adrenoceptor beta 2 surface; beta-2 adrenoceptor; beta-2 adrenoreceptor; catecholamine receptor
• UniProt Protein Name: Beta-2 adrenergic receptor
• Protein Family: Beta-2 adrenergic receptor
• UniProt Gene Name: ADRB2
• UniProt Synonym Gene Names: ADRB2R; B2AR; Beta-2 adrenoceptor
• UniProt Entry Name: ADRB2_HUMAN

Uniprot Description

ADRB2: Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q31-q32

Cellular Component: integral to plasma membrane; lysosome; apical plasma membrane; plasma membrane; nucleus; endosome; receptor complex

Molecular Function: protein binding; protein homodimerization activity; potassium channel regulator activity; beta2-adrenergic receptor activity; norepinephrine binding; adenylate cyclase binding

Biological Process: arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; diet induced thermogenesis; negative regulation of multicellular organism growth; transmembrane receptor protein tyrosine kinase activation (dimerization); regulation of vasodilation; adenylate cyclase activation; regulation of sodium ion transport; positive regulation of bone mineralization; G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; positive regulation of protein ubiquitination; heat generation; negative regulation of smooth muscle contraction; brown fat cell differentiation; endosome to lysosome transport; positive regulation of transcription from RNA polymerase II promoter; response to cold; bone resorption; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure

Disease: Obesity; Asthma, Susceptibility To