Rabbit GNAS Polyclonal Antibody | anti-GNAS antibody

GNAS Antibody (N-Term)

Cat. Num. AAA9215820

• Gene Names: GNAS; AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; C20orf45
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Synonyms: GNAS; Polyclonal Antibody; GNAS Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Guanine nucleotide-binding protein G(s) subunit alpha isoforms short; Adenylate cyclase-stimulating G alpha protein; GNAS1; GSP; anti-GNAS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This GNAS antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 51-83 amino acids from human GNAS.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 394

• Applicable Applications for anti-GNAS antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:8000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-GNAS Antibody (N-Term) at 1:8000 dilutionLane 1: HepG2 whole cell lysateLane 2: Raji whole cell lysateLane 3: mouse brain lysateLane 4: rat brain lysateLane 5: human cerebellum lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-GNAS antibody

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(s) protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Stimulates the Ras signaling pathway via RAPGEF2.

Product Categories/Family for anti-GNAS antibody

Crown Antibodies; Cancer; Metabolism; Signal Transduction

References

Mattera R.,et al.FEBS Lett. 206:36-42(1986). Harris B.A.,et al.Nucleic Acids Res. 16:3585-3585(1988). Kozasa T.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:2081-2085(1988). Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. Kalnine N.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 4504047
• NCBI GeneID: 2778
• NCBI Accession #: NP_000507.1
• NCBI GenBank Nucleotide #: NM_000516.5
• UniProt Accession #: P63092; P04895; Q12927; Q14433; Q32P26; Q5JWD2; Q5JWD4; Q5JWD5; Q6NR75; Q6NXS0; A6NI00; E1P5G5; O95467; P84996; Q5JWF2
• Molecular Weight: 45665
• NCBI Official Full Name: protein GNAS isoform GNASL
• NCBI Official Synonym Full Names: GNAS complex locus
• NCBI Official Symbol: GNAS
• NCBI Official Synonym Symbols: AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; C20orf45
• NCBI Protein Information: protein ALEX; protein GNAS; protein SCG6 (secretogranin VI)
• UniProt Protein Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
• Protein Family: Protein
• UniProt Gene Name: GNAS
• UniProt Synonym Gene Names: GNAS1; GSP
• UniProt Entry Name: GNAS2_HUMAN

NCBI Description

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012]

Uniprot Description

G-alpha(s): a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein type: Oncoprotein; G protein, heterotrimeric; Vesicle; G protein, heterotrimeric alpha G(s); G protein; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: ruffle; transport vesicle; membrane; perinuclear region of cytoplasm; dendrite; cytoplasm; plasma membrane; extracellular region; heterotrimeric G-protein complex; trans-Golgi network membrane; intrinsic to membrane; nucleus; cytosol

Molecular Function: corticotropin-releasing hormone receptor 1 binding; GTPase activity; GTP binding; metal ion binding; adenylate cyclase activity; insulin-like growth factor receptor binding; ionotropic glutamate receptor binding; signal transducer activity; protein binding; mu-type opioid receptor binding; G-protein beta/gamma-subunit binding; beta-2 adrenergic receptor binding; D1 dopamine receptor binding

Biological Process: developmental growth; positive regulation of osteoclast differentiation; water transport; protein secretion; regulation of signal transduction; female pregnancy; embryonic hindlimb morphogenesis; G-protein signaling, adenylate cyclase activating pathway; intracellular transport; positive regulation of cAMP biosynthetic process; DNA methylation; tissue homeostasis; transmembrane transport; endochondral ossification; post-embryonic body morphogenesis; dopamine receptor, adenylate cyclase activating pathway; response to drug; sensory perception of chemical stimulus; negative regulation of multicellular organism growth; multicellular organism growth; sensory perception of smell; adenylate cyclase activation; embryonic cranial skeleton morphogenesis; positive regulation of osteoblast differentiation; cartilage development; energy reserve metabolic process; renal water homeostasis; cAMP biosynthetic process; cognition; blood coagulation; regulation of insulin secretion; positive regulation of GTPase activity

Disease: Osseous Heteroplasia, Progressive; Pseudohypoparathyroidism, Type Ic; Pseudopseudohypoparathyroidism; Mccune-albright Syndrome; Acth-independent Macronodular Adrenal Hyperplasia; Pseudohypoparathyroidism, Type Ia; Pseudohypoparathyroidism, Type Ib; Pituitary Adenoma, Growth Hormone-secreting